Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ATP5H CRISPR Activation Plasmid (h): sc-403948-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ATP5H CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- ATP5H CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by ATP5H CRISPR Activation Plasmid (h) and ATP5H CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the ATP5H transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ATP5H Antibody (E-1): sc-515915
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ATP5H CRISPR Activation Plasmid (h) | sc-403948-ACT | 20 µg | $397.00 |
ATP5H encodes a small structural subunit of mitochondrial ATP synthase (Complex V) that supports assembly and stability of the F0 sector required for proton-coupled ATP production. By contributing to oxidative phosphorylation, ATP5H influences cellular energy homeostasis, mitochondrial membrane potential, and redox balance, with downstream effects on metabolism-dependent signaling and stress responses. Altered expression or function of ATP synthase subunits is frequently linked to mitochondrial dysfunction phenotypes, including impaired bioenergetics and heightened sensitivity to oxidative stress, which are recurrent features across neuromuscular and metabolic disorders. As a mitochondrial bioenergetics node, ATP5H is relevant for studies of respiratory chain regulation, mitochondrial quality control, and metabolic reprogramming in disease-relevant cell states.
ATP5H CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous ATP5H expression without altering the underlying DNA sequence.
ATP5H CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the ATP5H locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the ATP5H transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous ATP5H expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native ATP5H locus and enabling the study of ATP5H-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of ATP5H pathway restoration in tumor cells with silenced or reduced ATP5H expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.