Date published: 2025-9-7
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ATP-citrate synthase Antibody (5F8D11) - Western Blotting - Image 368288
ATP-柠檬酸合成酶抗体 (5F8D11): sc-517267. Jurkat (A), HeLa (B), Raji (C), A-431 (D), K-562 (E) 和 3T3-L1 (F) 全细胞裂解液中 ATP 柠檬酸合成酶表达的 Western 印迹分析.
ATP-柠檬酸合成酶抗体 (5F8D11): sc-517267. Jurkat (A), HeLa (B), Raji (C), A-431 (D), K-562 (E) 和 3T3-L1 (F) 全细胞裂解液中 ATP 柠檬酸合成酶表达的 Western 印迹分析.

ATP-citrate synthase 抗体 (5F8D11): sc-517267

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说明书
  • ATP-citrate synthase 抗体 5F8D11 是小鼠单克隆 IgG1 κ，ATP-citrate synthase抗体, 在12篇文献中引用，规格为200 µg/ml
  • 免疫human物种的的氨基酸306-502
  • ATP-citrate synthase 抗体 (5F8D11) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 ATP-citrate synthase
  • 抗ATP-citrate synthase抗体（5F8D11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ATP-citrate synthase (5F8D11): sc-517267的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 ATP-citrate synthase 抗体 (5F8D11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ATP-citrate synthase 抗体 (5F8D11) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Citrate synthase 抗体

ATP-citrate synthase Antibody (5F8D11) 是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的 ATP-citrate synthase 蛋白。抗ATP-柠檬酸合成酶抗体（5F8D11）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。ATP-柠檬酸合成酶，也称为ATP-柠檬酸裂解酶或柠檬酸裂解酶，是一种细胞质同源四聚体，属于琥珀酸/苹果酸CoA结合酶家族，其基因位于17q12-q21染色体上。这种酶在细胞代谢中起着至关重要的作用，催化柠檬酸盐和辅酶A转化为乙酰辅酶A和草酰乙酸。乙酰辅酶A是脂肪酸和胆固醇生物合成的重要底物，因此ATP-柠檬酸盐合成酶对于能量产生和脂质代谢至关重要。此外，ATP-柠檬酸盐合成酶在神经组织乙酰胆碱的生物合成中也很重要，这凸显了它在神经传递和整体大脑功能中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ATP-citrate synthase 参考文献:

  1. 固醇调节元件结合蛋白对 ATP 柠檬酸-赖氨酸酶基因的转录调控。  |  Sato, R., et al. 2000. J Biol Chem. 275: 12497-502. PMID: 10777536
  2. 大鼠 ATP 柠檬酸-赖氨酸酶基因启动子中顺式作用元件的特征。  |  Moon, YA., et al. 2002. Exp Mol Med. 34: 60-8. PMID: 11989980
  3. 在原发性脂肪细胞中发现 ATP 柠檬酸溶解酶是蛋白激酶 B (Akt) 的底物。  |  Berwick, DC., et al. 2002. J Biol Chem. 277: 33895-900. PMID: 12107176
  4. 拟南芥细胞质乙酰-CoA 由 ATP 柠檬酸裂解酶生成的反向遗传特征。  |  Fatland, BL., et al. 2005. Plant Cell. 17: 182-203. PMID: 15608338
  5. 补充柠檬酸钾镁对白细胞细胞膜 ATP 柠檬酸酶和线粒体丙酮酸酶活性的影响：肾脏柠檬酸代谢的窗口。  |  Tosukhowong, P., et al. 2005. Int J Urol. 12: 140-4. PMID: 15733107
  6. 利用 iTRAQ 对硒蛋白 F 基因剔除小鼠的肝脏蛋白质组分析：硒蛋白 F 在新陈代谢和疾病中的作用。  |  Zheng, X., et al. 2020. J Proteomics. 215: 103653. PMID: 31958640
  7. 肝细胞癌中线粒体乙酰化受乙酰基可用性的影响。  |  Hu, X., et al. 2021. Mitochondrion. 60: 150-159. PMID: 34375734
  8. 钠依赖性 SLC13 转运体抑制剂在各种代谢紊乱中的作用。  |  Akhtar, MJ., et al. 2023. Mol Cell Biochem. 478: 1669-1687. PMID: 36495372
  9. 2-Bromoethanesulfonic sodium 对甲烷生成的影响：甲烷菌的新陈代谢和群落结构。  |  Qiu, S., et al. 2023. Water Res. 230: 119527. PMID: 36580800
  10. Morusin，一种新型的 ACLY 抑制剂，通过 ROS 介导的线粒体自噬诱导肝细胞癌细胞凋亡。  |  Li, D., et al. 2024. Biomed Pharmacother. 180: 117510. PMID: 39341077
  11. 人 ATP:citrate lyase 的变异 cDNA 序列：从杆状病毒感染的昆虫细胞中克隆, 表达和纯化。  |  Lord, KA., et al. 1997. Protein Expr Purif. 9: 133-41. PMID: 9116495
  12. 小鼠 ATP 柠檬酸酶缺乏症  |  Beigneux, AP., et al. 2004. J Biol Chem. 279: 9557-64. PMID: 14662765

订购信息

产品名称产品编号规格价格数量收藏夹

ATP-citrate synthase 抗体 (5F8D11)

sc-517267
200 µg/ml
$316.00

ATP-citrate synthase (5F8D11): m-IgG Fc BP-HRP 套装

sc-531574
200 µg Ab; 10 µg BP
$354.00

ATP-citrate synthase (5F8D11): m-IgGκ BP-HRP 套装

sc-525302
200 µg Ab, 40 µg BP
$354.00

ATP-citrate synthase 抗体 (5F8D11) AC

sc-517267 AC
500 µg/ml, 25% agarose
$416.00

ATP-citrate synthase 抗体 (5F8D11) HRP

sc-517267 HRP
200 µg/ml
$316.00

ATP-citrate synthase 抗体 (5F8D11) FITC

sc-517267 FITC
200 µg/ml
$330.00

ATP-citrate synthase 抗体 (5F8D11) PE

sc-517267 PE
200 µg/ml
$343.00

ATP-citrate synthase 抗体 (5F8D11) Alexa Fluor® 488

sc-517267 AF488
200 µg/ml
$357.00

ATP-citrate synthase 抗体 (5F8D11) Alexa Fluor® 546

sc-517267 AF546
200 µg/ml
$357.00

ATP-citrate synthase 抗体 (5F8D11) Alexa Fluor® 594

sc-517267 AF594
200 µg/ml
$357.00

ATP-citrate synthase 抗体 (5F8D11) Alexa Fluor® 647

sc-517267 AF647
200 µg/ml
$357.00

ATP-citrate synthase 抗体 (5F8D11) Alexa Fluor® 680

sc-517267 AF680
200 µg/ml
$357.00

ATP-citrate synthase 抗体 (5F8D11) Alexa Fluor® 790

sc-517267 AF790
200 µg/ml
$357.00

Hi, How this Ab is different from citrate synthase Antibody (G-3): sc-390693. Do you have FCM standardized protocol for this Ab! Thanks

Asked by: Tube
Thank you for your question. These two antibodies are directed against different proteins. This mouse monoclonal, ATP-citrate synthase (5F8D11): sc-517267, is raised against the human protein derived from the ACLY gene product (Uniprot accession number P53396). And this different mouse monoclonal antibody, citrate synthase (G-3): sc-390693, is raised against the human protein derived from the CS gene product (Uniprot accession number O75390). A link to our standard recommended protocol for FCM is provided below. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-05-18

How much sample do you recommend loading on the gel for Western blot detection using ATP-citrate synthase (5F8D11): sc-517267?

Asked by: DefinitelyNotMatt
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from 功能很出色很棒,zhiliangyeshihenbangdi,以后会多多支持，很喜欢，发货速度也很快。。。。。。
Date published: 2022-05-19
Rated 5 out of 5 by from ATP citrate lyase (ACLY) antibody (5F8D11)sc-517267 is a good antibody for the identification of ACLY. The signal is clean, with no additional non-specific bands. The antibody was used at a 1:1000 dilution in milk 5%, on blots loaded with 40ug of HepG2 total extract protein.
Date published: 2021-04-19
Rated 5 out of 5 by from Strong band in liver samplesI tried the antibody in mouse liver samples and the band was very strong at ~120KDa aprox. I used: - 30µg of protein - 1h blocking - 1:1,000 dilution of the antibody in BSA 5%, over night at 4C - 1h secondary anti-mouse 1:10,000
Date published: 2018-02-25
Rated 4 out of 5 by from work for 293 cellsUsed product at 1:2000 on 293 cells, detection of ACLY at ~120kDa.
Date published: 2017-12-20
Rated 5 out of 5 by from Good featuresI applied for a sample of this antibody two months ago and I did WB(dilution 1:200), the result is good.
Date published: 2017-07-06
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ATP-citrate synthase 抗体 (5F8D11) is rated 4.8 out of 5 by 5.
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