ATIII Double Nickase Plasmid (m): sc-419222-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ATIII Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ATIII Double Nickase Plasmid (m) and ATIII Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Serpinc1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ATIII Antibody (H-7): sc-271987
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ATIII Double Nickase Plasmid (m) | sc-419222-NIC | 20 µg | $410.00 | |||
ATIII Double Nickase Plasmid (m2) | sc-419222-NIC-2 | 20 µg | $410.00 |
Serpinc1 encodes antithrombin III (ATIII), a secreted serine protease inhibitor that restrains coagulation by inactivating thrombin and factor Xa, with activity strongly potentiated by heparan sulfate and heparin. In mouse, ATIII contributes to vascular homeostasis and links coagulation to inflammatory signaling and endothelial function through regulation of protease-driven pathways. Disruption of ATIII activity is associated with hypercoagulable phenotypes and thrombotic susceptibility, making Serpinc1 a key gene for studying hemostasis and thromboinflammatory crosstalk. Serpinc1 is also relevant to hepatic synthesis and secretion pathways because ATIII is produced predominantly by hepatocytes and circulates as a plasma glycoprotein.
ATIII Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Serpinc1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Serpinc1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Serpinc1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Serpinc1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.