ATGL Double Nickase Plasmid (m): sc-426224-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ATGL Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ATGL Double Nickase Plasmid (m) and ATGL Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Pnpla2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ATGL Antibody (F-7): sc-365278
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ATGL Double Nickase Plasmid (m) | sc-426224-NIC | 20 µg | $410.00 | |||
ATGL Double Nickase Plasmid (m2) | sc-426224-NIC-2 | 20 µg | $410.00 |
Mouse Pnpla2 encodes adipose triglyceride lipase (ATGL), a rate-limiting enzyme that initiates triacylglycerol hydrolysis on lipid droplets to release fatty acids and diacylglycerol. ATGL activity coordinates cellular energy balance by supplying substrates for mitochondrial β-oxidation and modulating lipid droplet turnover, often acting in concert with cofactors such as ABHD5/CGI-58. Through its central role in neutral lipid catabolism, ATGL influences metabolic signaling and lipid homeostasis across adipose tissue, liver, heart, and skeletal muscle. Dysregulation of Pnpla2/ATGL is linked to pathological lipid accumulation and altered energy metabolism, making it a relevant target for studying lipid storage disorders and metabolic stress responses in vivo and in cultured mouse cells.
ATGL Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Pnpla2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Pnpla2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Pnpla2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Pnpla2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.