Atg9a Antibody (F-3): sc-518179
- Atg9a Antibody (F-3) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 561-839 mapping at the C-terminus of Atg9a of human origin
- recommended for detection of Atg9a of human origin by WB, IP, IF and ELISA
- Anti-Atg9a Antibody (F-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Atg9a Antibody (F-3) for WB applications. These reagents are now offered in bundles with Atg9a Antibody (F-3) (see ordering information below).
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Atg9a Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Atg9a of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Atg9a antibody (F-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Atg9a, also known as autophagy-related protein 9A or APG9-like 1, is a crucial multi-pass membrane protein consisting of 839 amino acids that plays a significant role in the autophagy process, which is essential for cellular homeostasis and the degradation of damaged organelles and proteins. Atg9a is primarily localized to the pre-autophagosomal structure (PAS), where Atg9a is involved in the formation of isolation membranes that eventually wrap around cytoplasmic components to create double-membrane autophagosomes. These autophagosomes then fuse with lysosomes for degradation, highlighting Atg9a′s importance in maintaining cellular health, especially during nutrient deprivation. Atg9a cycling between the Golgi apparatus and endosomes, along with Atg9a interaction with the autophagosome-specific marker LC3, underscores Atg9a′s critical function in starvation-induced autophagosome formation. Additionally, three isoforms of Atg9a arise from alternative splicing, which may contribute to Atg9a′s diverse functional roles in different cellular contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Atg9a Antibody (F-3) References:
- Endothelial nitric-oxide synthase antisense (NOS3AS) gene encodes an autophagy-related protein (APG9-like2) highly expressed in trophoblast. | Yamada, T., et al. 2005. J Biol Chem. 280: 18283-90. PMID: 15755735
- Starvation and ULK1-dependent cycling of mammalian Atg9 between the TGN and endosomes. | Young, AR., et al. 2006. J Cell Sci. 119: 3888-900. PMID: 16940348
- Atg9 trafficking in Mammalian cells. | Webber, JL., et al. 2007. Autophagy. 3: 54-6. PMID: 17102588
- Atg27 is required for autophagy-dependent cycling of Atg9. | Yen, WL., et al. 2007. Mol Biol Cell. 18: 581-93. PMID: 17135291
- Atg9 trafficking in autophagy-related pathways. | He, C. and Klionsky, DJ. 2007. Autophagy. 3: 271-4. PMID: 17329962
- Molecular machinery of autophagosome formation in yeast, Saccharomyces cerevisiae. | Suzuki, K. and Ohsumi, Y. 2007. FEBS Lett. 581: 2156-61. PMID: 17382324
- A cycling protein complex required for selective autophagy. | Legakis, JE., et al. 2007. Autophagy. 3: 422-32. PMID: 17426440
- Autophagosome formation: core machinery and adaptations. | Xie, Z. and Klionsky, DJ. 2007. Nat Cell Biol. 9: 1102-9. PMID: 17909521
- A novel link between autophagy and the ubiquitin-proteasome system. | Korolchuk, VI., et al. 2009. Autophagy. 5: 862-3. PMID: 19458478
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Atg9a Antibody (F-3) | sc-518179 | 200 µg/ml | $316.00 | |||
Atg9a Antibody (F-3): m-IgG Fc BP-HRP Bundle | sc-550712 | 200 µg Ab; 10 µg BP | $354.00 | |||
Atg9a Antibody (F-3): m-IgG1 BP-HRP Bundle | sc-550713 | 200 µg Ab; 20 µg BP | $354.00 | |||
Atg9a Antibody (F-3) AC | sc-518179 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Atg9a Antibody (F-3) HRP | sc-518179 HRP | 200 µg/ml | $316.00 | |||
Atg9a Antibody (F-3) FITC | sc-518179 FITC | 200 µg/ml | $330.00 | |||
Atg9a Antibody (F-3) PE | sc-518179 PE | 200 µg/ml | $343.00 | |||
Atg9a Antibody (F-3) Alexa Fluor® 488 | sc-518179 AF488 | 200 µg/ml | $357.00 | |||
Atg9a Antibody (F-3) Alexa Fluor® 546 | sc-518179 AF546 | 200 µg/ml | $357.00 | |||
Atg9a Antibody (F-3) Alexa Fluor® 594 | sc-518179 AF594 | 200 µg/ml | $357.00 | |||
Atg9a Antibody (F-3) Alexa Fluor® 647 | sc-518179 AF647 | 200 µg/ml | $357.00 | |||
Atg9a Antibody (F-3) Alexa Fluor® 680 | sc-518179 AF680 | 200 µg/ml | $357.00 | |||
Atg9a Antibody (F-3) Alexa Fluor® 790 | sc-518179 AF790 | 200 µg/ml | $357.00 |