ATF-1/ATF1 Antibody (C41-5.1): sc-243

ATF-1 Antibody (C41-5.1) is a mouse monoclonal IgA antibody that detects ATF-1 p35 protein of mouse, rat, and human origin through western blotting (WB) and immunoprecipitation (IP). ATF-1 (C41-5.1) antibody is offered in a non-conjugated form, allowing for versatile applications in research. ATF-1 monoclonal antibody (C41-5.1) recognizes a crucial member of the ATF/CREB transcription factor family, which plays a significant role in regulating eukaryotic gene transcription. This family, which includes CREB-1 and ATF-2, binds to the cAMP response element (CRE) to modulate gene expression in response to various signaling pathways. ATF-1 is particularly important for NOX1 induction, a protein involved in reactive oxygen species production, which are essential for various cellular processes including immune response and cell signaling. ATF-1 binding to CRE and mediating protein kinase A (PKA)-induced stimulation of CRE-reporter genes highlights a role in cellular responses to stress and hormonal signals. ATF-2, another member of this family, can form homodimers and heterodimers with c-Jun, further influencing CRE-dependent transcription. Phosphorylation of ATF-2 by stress-activated kinases is critical for transcriptional activation, demonstrating intricate regulatory mechanisms that govern gene expression. ATF-1 (C41-5.1) monoclonal antibody serves as a valuable tool for researchers studying complex roles of transcription factors in cellular signaling and gene regulation.

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ATF-1/ATF1 Antibody (C41-5.1) References:

1. cAMP-independent activation of the adenovirus type 12 E2 promoter correlates with the recruitment of CREB-1/ATF-1, E1A(12S), and CBP to the E2-CRE.  |  Fax, P., et al. 2000. J Biol Chem. 275: 8911-20. PMID: 10722738
2. Analysis of the Epstein-Barr virus (EBV) latent membrane protein 1 (LMP-1) gene and promoter in Hodgkin's disease isolates: selection against EBV variants with mutations in the LMP-1 promoter ATF-1/CREB-1 binding site.  |  Sandvej, K., et al. 2000. Mol Pathol. 53: 280-8. PMID: 11091852
3. EWS-ATF-1 chimeric protein in soft tissue clear cell sarcoma associates with CREB-binding protein and interferes with p53-mediated trans-activation function.  |  Fujimura, Y., et al. 2001. Oncogene. 20: 6653-9. PMID: 11709699
4. ATF-1 mediates protease-activated receptor-1 but not receptor tyrosine kinase-induced DNA synthesis in vascular smooth muscle cells.  |  Ghosh, SK., et al. 2002. J Biol Chem. 277: 21325-31. PMID: 11925444
5. Identification of target genes for EWS/ATF-1 chimeric transcription factor.  |  Jishage, M., et al. 2003. Oncogene. 22: 41-9. PMID: 12527906
6. The cAMP-regulated enhancer-binding protein ATF-1 activates transcription in response to cAMP-dependent protein kinase A.  |  Rehfuss, RP., et al. 1991. J Biol Chem. 266: 18431-4. PMID: 1655749
7. ATF-1 transcription factor regulates the expression of ccg-1 and cat-1 genes in response to fludioxonil under OS-2 MAP kinase in Neurospora crassa.  |  Yamashita, K., et al. 2008. Fungal Genet Biol. 45: 1562-9. PMID: 18948219
8. The transcription factors ATF-1 and CREB-1 bind constitutively to the hypoxia-inducible factor-1 (HIF-1) DNA recognition site.  |  Kvietikova, I., et al. 1995. Nucleic Acids Res. 23: 4542-50. PMID: 8524640
9. The EWS-ATF-1 gene involved in malignant melanoma of soft parts with t(12;22) chromosome translocation, encodes a constitutive transcriptional activator.  |  Fujimura, Y., et al. 1996. Oncogene. 12: 159-67. PMID: 8552387
10. The CREB, ATF-1, and ATF-2 transcription factors from bovine leukemia virus-infected B lymphocytes activate viral expression.  |  Adam, E., et al. 1996. J Virol. 70: 1990-9. PMID: 8627725

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ATF-1/ATF1 Antibody (C41-5.1) has 104 citations in a variety of scientific publications.

Primary antibodies like Anti-ATF-1/ATF1 Antibody (C41-5.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ATF-1/ATF1 Antibody (C41-5.1) Specifications

Alternate Product Name: ATF1

Product Clone Name: C41-5.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgA kappa light chain

Amino Acids:

Epitope Target: ATF-1

Epitope Species: human

Product Reactivity/Detection: ATF-1 p35

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgA kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 104 citations in various scientific publications

Available Conjugates/Forms for ATF-1/ATF1 Antibody (C41-5.1): X: TransCruz Reagent

The preferred CREB1 antibody is CREB1 Antibody (D-12): sc-377154.