ATBF1 Antibody (3B1): sc-517126
- ATBF1 Antibody (3B1) is a mouse monoclonal IgG1 κ ATBF1 antibody provided at 100 µg/ml
- raised against amino acids 2811-2910 representing partial length ATBF1 of human origin
- recommended for detection of ATBF1 of mouse, rat and human origin by WB, IP, IF and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ATBF1 Antibody (3B1). This work is in progress.
ATBF1 Antibody (3B1) is a mouse monoclonal IgG1 kappa light chain antibody that detects ATBF1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ATBF1 antibody (3B1) is available as the non-conjugated form. The AT-motif binding factor 1 (ATBF1) plays a crucial role in gene regulation by binding to the AT-rich core sequence element in the human alpha-fetoprotein enhancer, which is essential for proper embryonic development and cellular differentiation. Notably, ATBF1 exists in two isoforms, ATBF1-A and ATBF1-B, generated through alternative splicing. The ATBF1-A isoform features a unique 920-amino acid N-terminal extension that enhances DNA-binding capabilities and exhibits transcriptional repressor activity, particularly inhibiting the expression of brush border enzyme aminopeptidase-N in the small intestine. Both isoforms contain four DNA-binding homeobox domains, with ATBF1-A possessing 23 zinc finger motifs and ATBF1-B having 18, which are critical for interaction with specific DNA sequences. Additionally, ATBF1 is involved in ATPase activity, which is DNA/RNA-dependent and requires the presence of both homeobox domains and zinc finger motifs. ATBF1 monoclonal antibody (3B1) is particularly valuable for research in transcription regulation and cellular signaling pathways, as ATBF1 is highly expressed in spleen and brain tissues, with the gene encoding human ATBF1 mapping to chromosome 16q22.3-q23.1.
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ATBF1 Antibody (3B1) References:
- Regulation of the alpha-fetoprotein gene by the isoforms of ATBF1 transcription factor in human hepatoma. | Ninomiya, T., et al. 2002. Hepatology. 35: 82-7. PMID: 11786962
- A human alpha-fetoprotein enhancer-binding protein, ATBF1, contains four homeodomains and seventeen zinc fingers. | Morinaga, T., et al. 1991. Mol Cell Biol. 11: 6041-9. PMID: 1719379
- ATBF1, a multiple-homeodomain zinc finger protein, selectively down-regulates AT-rich elements of the human alpha-fetoprotein gene. | Yasuda, H., et al. 1994. Mol Cell Biol. 14: 1395-401. PMID: 7507206
- Cloning and characterization of an ATBF1 isoform that expresses in a neuronal differentiation-dependent manner. | Miura, Y., et al. 1995. J Biol Chem. 270: 26840-8. PMID: 7592926
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ATBF1 Antibody (3B1) | sc-517126 | 100 µg/ml | $322.00 |