ASM Antibody (4H2): sc-293189

ASM Antibody (4H2) is a mouse monoclonal IgG2a kappa light chain antibody that detects ASM protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-ASM antibody (4H2) is available as a non-conjugated isotype antibody. Acid sphingomyelinase (ASM) plays a crucial role in lipid metabolism by hydrolyzing sphingomyelin into ceramide and phosphocholine, which are essential for maintaining cellular membrane integrity and signaling pathways. ASM deficiency leads to severe conditions such as type A and type B Niemann-Pick disease; type A is characterized by neurodegeneration and typically results in death by age three, while type B presents a non-neuropathic phenotype with a later onset. ASM gene encodes three protein isoforms, with ASM-1 being the only catalytically active enzyme, highlighting the importance of this specific isoform in disease pathology. During the differentiation of monocytic cells, ASM expression is significantly upregulated through the synergistic actions of transcription factors AP-2 and Sp1, underscoring the regulatory mechanisms that control ASM levels in response to cellular cues.

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ASM Antibody (4H2) References:

1. Transcription factors Sp1 and AP-2 mediate induction of acid sphingomyelinase during monocytic differentiation.  |  Langmann, T., et al. 1999. J Lipid Res. 40: 870-80. PMID: 10224156
2. Identification and expression of five mutations in the human acid sphingomyelinase gene causing types A and B Niemann-Pick disease. Molecular evidence for genetic heterogeneity in the neuronopathic and non-neuronopathic forms.  |  Takahashi, T., et al. 1992. J Biol Chem. 267: 12552-8. PMID: 1618760
3. Human acid sphingomyelinase. Isolation, nucleotide sequence and expression of the full-length and alternatively spliced cDNAs.  |  Schuchman, EH., et al. 1991. J Biol Chem. 266: 8531-9. PMID: 1840600
4. Niemann-Pick disease: a frequent missense mutation in the acid sphingomyelinase gene of Ashkenazi Jewish type A and B patients.  |  Levran, O., et al. 1991. Proc Natl Acad Sci U S A. 88: 3748-52. PMID: 2023926
5. Disease manifestations and burden of illness in patients with acid sphingomyelinase deficiency (ASMD).  |  McGovern, MM., et al. 2017. Orphanet J Rare Dis. 12: 41. PMID: 28228103
6. Acid sphingomyelinase from human urine: purification and characterization.  |  Quintern, LE., et al. 1987. Biochim Biophys Acta. 922: 323-36. PMID: 2825797
7. Role of acid sphingomyelinase-induced ceramide generation in response to radiation.  |  Sharma, D. and Czarnota, GJ. 2019. Oncotarget. 10: 6-7. PMID: 30713596
8. Acid Sphingomyelinase-Ceramide System in Bacterial Infections.  |  Li, C., et al. 2019. Cell Physiol Biochem. 52: 280-301. PMID: 30816675
9. Acid sphingomyelinase promotes SGK1-dependent vascular calcification.  |  Luong, TTD., et al. 2021. Clin Sci (Lond). 135: 515-534. PMID: 33479769
10. The acid sphingomyelinase/ceramide system in COVID-19.  |  Kornhuber, J., et al. 2022. Mol Psychiatry. 27: 307-314. PMID: 34608263
11. Polyphyllin D punctures hypertrophic lysosomes to reverse drug resistance of hepatocellular carcinoma by targeting acid sphingomyelinase.  |  Wang, Y., et al. 2023. Mol Ther. 31: 2169-2187. PMID: 37211762
12. Acid sphingomyelinase mediates ferroptosis induced by high glucose via autophagic degradation of GPX4 in type 2 diabetic osteoporosis.  |  Du, YX., et al. 2023. Mol Med. 29: 125. PMID: 37710183

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ASM Antibody (4H2) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-ASM Antibody (4H2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ASM Antibody (4H2) Specifications

Alternate Product Name: Acid Sphingomyelinase

Product Clone Name: 4H2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-364

Epitope Target: ASM

Epitope Species: human

Product Reactivity/Detection: ASM

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for ASM Antibody (4H2): N/A