ASC/TMS1/PYCARD Double Nickase Plasmid (h): sc-400151-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ASC/TMS1/PYCARD Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ASC/TMS1/PYCARD Double Nickase Plasmid (h) and ASC/TMS1/PYCARD Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PYCARD. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ASC/TMS1/PYCARD Antibody (B-3): sc-514414
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ASC/TMS1/PYCARD Double Nickase Plasmid (h) | sc-400151-NIC | 20 µg | $410.00 | |||
ASC/TMS1/PYCARD Double Nickase Plasmid (h2) | sc-400151-NIC-2 | 20 µg | $410.00 |
PYCARD, also known as ASC/TMS1, encodes an adaptor protein that nucleates inflammasome assembly by linking sensor proteins such as NLRP3 and AIM2 to CASP1 through its PYD and CARD domains. This scaffolding function enables proximity-induced caspase-1 activation, leading to cleavage of pro-IL-1β and pro-IL-18 and induction of pyroptotic cell death via gasdermin D, positioning ASC at the center of innate immune and stress-response signaling. In human cells, PYCARD participates in pathogen recognition, sterile inflammation, and cell death pathways that shape macrophage and epithelial immune responses. Dysregulated PYCARD/ASC signaling is implicated in chronic inflammatory disorders, neuroinflammation, metabolic disease, and tumor-associated inflammation, making it a common target for mechanistic studies of cytokine maturation and inflammasome dynamics.
ASC/TMS1/PYCARD Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PYCARD locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PYCARD. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PYCARD function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PYCARD-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.