ARMCX3 Double Nickase Plasmid (h): sc-409945-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ARMCX3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ARMCX3 Double Nickase Plasmid (h) and ARMCX3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ARMCX3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ARMCX3 Antibody (A-8): sc-393752
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARMCX3 Double Nickase Plasmid (h) | sc-409945-NIC | 20 µg | $410.00 | |||
ARMCX3 Double Nickase Plasmid (h2) | sc-409945-NIC-2 | 20 µg | $410.00 |
ARMCX3 (armadillo repeat containing, X-linked 3) encodes a mitochondrial outer membrane–associated protein implicated in neuronal development and organelle homeostasis. ARMCX3 participates in regulating mitochondrial trafficking, dynamics, and quality control, processes that influence cellular bioenergetics and stress signaling. Through these functions, ARMCX3 is relevant to pathways linking mitochondrial distribution with survival and differentiation programs, including contexts where altered mitochondrial behavior contributes to neurodegenerative and cancer-related phenotypes. Dysregulation of ARMCX3 expression or function has been associated with changes in proliferation, apoptosis susceptibility, and migration-related signaling, making it a useful target for mechanistic studies of mitochondrial control in human cells.
ARMCX3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ARMCX3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ARMCX3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ARMCX3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ARMCX3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.