ARL13B Double Nickase Plasmid (h): sc-417325-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ARL13B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ARL13B Double Nickase Plasmid (h) and ARL13B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ARL13B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ARL13B Antibody (C-5): sc-515784
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARL13B Double Nickase Plasmid (h) | sc-417325-NIC | 20 µg | $410.00 | |||
ARL13B Double Nickase Plasmid (h2) | sc-417325-NIC-2 | 20 µg | $410.00 |
ARL13B encodes a cilia-enriched small Arf-like GTPase that supports primary cilium assembly, stability, and ciliary membrane identity, thereby coordinating trafficking of signaling components within the ciliary compartment. ARL13B contributes to the organization of axonemal architecture and regulates cilia-dependent pathways including Sonic hedgehog signaling, with downstream effects on developmental patterning and cellular differentiation programs. Disruption of ARL13B perturbs ciliogenesis and signal transduction, linking ARL13B dysfunction to ciliopathy-relevant phenotypes and neurodevelopmental disorders such as Joubert syndrome. As a ciliary GTPase, ARL13B is widely used as a marker and mechanistic node for studying organelle biogenesis, membrane trafficking, and pathway compartmentalization in human cells.
ARL13B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ARL13B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ARL13B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ARL13B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ARL13B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.