ARHGEF5 Antibody (4-YD14): sc-134269

ARHGEF5 Antibody (4-YD14) is a mouse monoclonal IgG2a kappa light chain antibody that detects ARHGEF5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-ARHGEF5 antibody (4-YD14) is available as a non-conjugated format. ARHGEF5, also known as Rho guanine nucleotide exchange factor 5, P60, or transforming immortalized mammary oncogene, is a 1597 amino acid cytoplasmic protein that plays a crucial role in cellular signaling pathways. ARHGEF5 (4-YD14) antibody recognizes a protein primarily involved in regulating Rho GTPases, which are essential for various cellular processes, including cytoskeletal organization, cell migration, and proliferation. ARHGEF5′s ability to interact with G proteins and initiate Rho-dependent signaling cascades underscores its importance in responding to external stimuli, particularly those mediated by G-protein coupled receptors. ARHGEF5 contains distinct structural domains, including one pleckstrin homology (PH) domain, one Dbl homology (DH) domain, and one Src homology 3 (SH3) domain, which facilitate interactions with other proteins and contribute to functional versatility. ARHGEF5 exists in two isoforms generated through alternative splicing and is predominantly expressed in tissues such as pancreas, liver, kidney, placenta, and lung, highlighting potential significance in both normal physiology and disease states.

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ARHGEF5 Antibody (4-YD14) References:

1. Structural basis for the selective activation of Rho GTPases by Dbl exchange factors.  |  Snyder, JT., et al. 2002. Nat Struct Biol. 9: 468-75. PMID: 12006984
2. Regulation of tolerance in the respiratory tract: TIM-1, hygiene, and the environment.  |  Umetsu, DT. and Dekruyff, RH. 2004. Ann N Y Acad Sci. 1029: 88-93. PMID: 15681748
3. Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules.  |  Zhang, Y., et al. 2005. Mol Cell Proteomics. 4: 1240-50. PMID: 15951569
4. Regulation of immature dendritic cell migration by RhoA guanine nucleotide exchange factor Arhgef5.  |  Wang, Z., et al. 2009. J Biol Chem. 284: 28599-606. PMID: 19713215
5. The guanine nucleotide exchange factor Arhgef5 plays crucial roles in Src-induced podosome formation.  |  Kuroiwa, M., et al. 2011. J Cell Sci. 124: 1726-38. PMID: 21525037
6. Targeting rho guanine nucleotide exchange factor ARHGEF5/TIM with auto-inhibitory peptides in human breast cancer.  |  Huang, O., et al. 2015. Amino Acids. 47: 1239-46. PMID: 25743165
7. The Rho guanine nucleotide exchange factor ARHGEF5 promotes tumor malignancy via epithelial-mesenchymal transition.  |  Komiya, Y., et al. 2016. Oncogenesis. 5: e258. PMID: 27617642
8. Arhgef5 Binds α-Dystrobrevin 1 and Regulates Neuromuscular Junction Integrity.  |  Bernadzki, KM., et al. 2020. Front Mol Neurosci. 13: 104. PMID: 32587503
9. Ephexin3/ARHGEF5 Together with Cell Migration Signaling Partners within the Tumor Microenvironment Define Prognostic Transcriptional Signatures in Multiple Cancer Types.  |  Juan-Guadarrama, DG., et al. 2023. Int J Mol Sci. 24: PMID: 38003617
10. High expression of ARHGEF5 predicts unfavorable prognosis in acute myeloid leukemia.  |  Xu, H., et al. 2024. Discov Oncol. 15: 491. PMID: 39331192
11. Assignment of the human TIM proto-oncogene to 7q33-->q35.  |  Takai, S., et al. 1995. Cancer Genet Cytogenet. 83: 87-9. PMID: 7656213
12. Expression cDNA cloning of a novel oncogene with sequence similarity to regulators of small GTP-binding proteins.  |  Chan, AM., et al. 1994. Oncogene. 9: 1057-63. PMID: 8134109

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ARHGEF5 Antibody (4-YD14) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-ARHGEF5 Antibody (4-YD14) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ARHGEF5 Antibody (4-YD14) Specifications

Alternate Product Name: ARHGEF5

Product Clone Name: 4-YD14

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-520

Epitope Target: ARHGEF5

Epitope Species: human

Product Reactivity/Detection: ARHGEF5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for ARHGEF5 Antibody (4-YD14): N/A