ArgBP2 Double Nickase Plasmid (h): sc-405716-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ArgBP2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ArgBP2 Double Nickase Plasmid (h) and ArgBP2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SORBS2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ArgBP2 Antibody (B-11): sc-514671
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ArgBP2 Double Nickase Plasmid (h) | sc-405716-NIC | 20 µg | $410.00 | |||
ArgBP2 Double Nickase Plasmid (h2) | sc-405716-NIC-2 | 20 µg | $410.00 |
SORBS2 encodes ArgBP2, an adaptor protein enriched in striated muscle and other mechanically active tissues that couples actin cytoskeletal remodeling to signal transduction. ArgBP2 contains SH3 domains that scaffold proline-rich partners and integrates inputs from kinases and adhesion complexes to regulate stress fiber organization, focal adhesion dynamics, and sarcomeric architecture. Through interactions with cytoskeletal regulators and Abl-family signaling components, SORBS2 contributes to pathways controlling cell shape, migration, and contractile function. Altered SORBS2 expression or network connectivity has been associated with cardiac and muscle phenotypes and is studied in the context of cardiomyopathy-related remodeling and cytoskeletal dysregulation.
ArgBP2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SORBS2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SORBS2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SORBS2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SORBS2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.