ARF5 Antibody (14-07): sc-81893

ARF5 Antibody (14-07) is a mouse monoclonal IgG1 kappa light chain antibody that detects ARF5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ARF5 antibody (14-07) is available as a non-conjugated format. ADP-ribosylation factor 5 (ARF5) is a member of the ARF family, which plays a crucial role in regulating vesicle trafficking and signal transduction within eukaryotic cells. ARF5 is primarily located in the Golgi apparatus, where ARF5 is essential for maintaining organelle integrity and facilitating the assembly of coat proteins vital for vesicle formation. This localization allows ARF5 to coordinate interactions between spectrin and Golgi membranes, as well as mediate association of actin with the Golgi through localization of Rho family G-proteins, such as Rac and CDC42, along with WASP/Arp2/3 complexes. ARF5 functions critically in cellular processes such as membrane trafficking, which impacts various physiological functions and cellular responses. ARF5 belongs to class II of the ARF protein family alongside ARF4, while class I includes ARF1, ARF2, and ARF3, and class III is represented by ARF6. Each class shares a common gene organization, highlighting evolutionary conservation and functional significance in cellular dynamics.

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ARF5 Antibody (14-07) References:

1. Activated ADP-ribosylation factor assembles distinct pools of actin on golgi membranes.  |  Fucini, RV., et al. 2000. J Biol Chem. 275: 18824-9. PMID: 10777475
2. The gamma-subunit of the coatomer complex binds Cdc42 to mediate transformation.  |  Wu, WJ., et al. 2000. Nature. 405: 800-4. PMID: 10866202
3. Golgi vesicle proteins are linked to the assembly of an actin complex defined by mAbp1.  |  Fucini, RV., et al. 2002. Mol Biol Cell. 13: 621-31. PMID: 11854417
4. The amino terminus of ADP-ribosylation factor (ARF) 1 is essential for interaction with Gs and ARF GTPase-activating protein.  |  Randazzo, PA., et al. 1994. J Biol Chem. 269: 29490-4. PMID: 7961931
5. Structure of the human ADP-ribosylation factor 1 complexed with GDP.  |  Amor, JC., et al. 1994. Nature. 372: 704-8. PMID: 7990966
6. Mammalian Cdc42 is a brefeldin A-sensitive component of the Golgi apparatus.  |  Erickson, JW., et al. 1996. J Biol Chem. 271: 26850-4. PMID: 8900167
7. ADP ribosylation factor regulates spectrin binding to the Golgi complex.  |  Godi, A., et al. 1998. Proc Natl Acad Sci U S A. 95: 8607-12. PMID: 9671725

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ARF5 Antibody (14-07) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-ARF5 Antibody (14-07) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ARF5 Antibody (14-07) Specifications

Alternate Product Name: ARF5

Product Clone Name: 14-07

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 81-180

Epitope Target: ARF5

Epitope Species: human

Product Reactivity/Detection: ARF5 and additional ARF family GTP binding proteins (significant homology with ARF4, ARF3 and ARF1)

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for ARF5 Antibody (14-07): N/A