ARF3 Double Nickase Plasmid (h): sc-402706-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ARF3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ARF3 Double Nickase Plasmid (h) and ARF3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ARF3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ARF3 Antibody (41): sc-135841
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARF3 Double Nickase Plasmid (h) | sc-402706-NIC | 20 µg | $410.00 | |||
ARF3 Double Nickase Plasmid (h2) | sc-402706-NIC-2 | 20 µg | $410.00 |
ADP-ribosylation factor 3 (ARF3) is a small GTPase of the ARF family that regulates vesicular trafficking by controlling coat protein recruitment, membrane curvature, and phospholipid metabolism at the Golgi and endosomal system. Cycling between GDP- and GTP-bound states links ARF3 to secretory and endocytic transport, including COPI-mediated retrograde trafficking and coordination of cargo sorting. Through its roles in membrane dynamics and signaling at intracellular membranes, ARF3 influences processes such as receptor recycling, protein secretion, and cytoskeletal organization. Dysregulated ARF family signaling and trafficking pathways have been associated with altered cell migration, proliferation, and stress responses, making ARF3 a useful target for mechanistic studies of trafficking-related phenotypes in disease-relevant models.
ARF3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ARF3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ARF3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ARF3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ARF3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.