Date published: 2026-4-5

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ARF1 Antibody (ARFS 5F2/2): sc-53169

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Datasheets
  • ARF1 Antibody (ARFS 5F2/2) is a mouse monoclonal mouse IgM κ κ provided at 200 µg/ml
  • raised against amino acids 174-180 of ARF1 human origin
  • recommended for detection of ARF1 of mouse, rat and human origin by WB, IP, IF and ELISA
  • See ARF1 (ARFS 1A9/5): sc-53168 for ARF1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ARF1 Antibody (ARFS 5F2/2). This work is in progress.
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    ARF1 Antibody (ARFS 5F2/2) is a mouse monoclonal IgM antibody that detects ARF1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ARF1 is a crucial member of the ADP-ribosylation factor family, which plays a significant role in vesicular transport and membrane trafficking within eukaryotic cells. ARF1 is primarily located at the Golgi membrane, where ARF1 is involved in the recruitment of coatomer protein complex I (COPI), a heptameric protein essential for the formation of transport vesicles. The interaction between ARF1 and COPI is vital for maintaining organelle integrity and facilitating the proper distribution of proteins and lipids throughout the cell. Additionally, ARF1 is regulated by the binding and hydrolysis of guanosine triphosphate (GTP), which activates ARF1 function and allows for effective mediation of membrane dynamics. The ability of ARF1 to reversibly associate with membranes and interact with specific phospholipids underscores ARF1′s importance in cellular processes, making anti-ARF1 antibody (ARFS 5F2/2) an invaluable tool for researchers studying membrane trafficking and related pathways.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    ARF1 Antibody (ARFS 5F2/2)

    sc-53169
    200 µg/ml
    $322.00

    What is the recommended fixation method for immunofluorescence staining with ARF1 (ARFS 5F2/2): sc-53169 monoclonal antibody?

    Asked by: TinTin
    Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
    Answered by: Technical Support
    Date published: 2017-02-28
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    Rated 3 out of 5 by from Band of interest observed in WB in mouseBand of interest observed in WB in mouse ARF1 transfected 293T whole cell lysate. -SCBT QC
    Date published: 2015-06-06
    Rated 3 out of 5 by from Nice immunofluorescence cytoplasmic stainingNice immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
    Date published: 2015-03-02
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    ARF1 Antibody (ARFS 5F2/2) is rated 3.0 out of 5 by 2.
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