ARA70 (h): 293T Lysate: sc-113567

ARA70 (h): 293T Lysate is derived from the 293T cell line, a human embryonic kidney cell line that has been modified to express the SV40 large T antigen, enhancing its transfection efficiency and protein expression capabilities. This lysate is enriched with human Androgen Receptor Associated Protein 70 (ARA70), a coactivator known to enhance the transcriptional activity of the androgen receptor (AR). ARA70 interacts with AR and other nuclear receptors, playing a crucial role in regulating gene expression in response to androgens. In research, ARA70 (h): 293T Lysate is used to study the mechanisms of androgen receptor signaling and the role of coactivators in gene regulation. Researchers employ this lysate in Western blotting to detect and quantify ARA70 expression levels under different experimental conditions, providing insights into its regulation and interaction with other proteins. The lysate is also utilized in co-immunoprecipitation assays to identify and characterize protein-protein interactions involving ARA70, helping to explain its role in the androgen receptor signaling pathway. Additionally, this lysate is valuable in transcriptional activity assays to measure the impact of ARA70 on androgen receptor-mediated gene expression. Researchers explore how ARA70 enhances AR activity and influences the expression of target genes, contributing to our understanding of steroid hormone signaling and its regulatory networks. The use of ARA70 (h): 293T Lysate allows for detailed investigations into the molecular mechanisms underlying androgen receptor function and the role of coactivators in gene transcription.