Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Aquaporin 9/AQP9 CRISPR/Cas9 KO Plasmid (h): sc-401298
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Aquaporin 9/AQP9 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Aquaporin 9/AQP9 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Aquaporin 9/AQP9 Antibody (G-3): sc-74409
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aquaporin 9/AQP9 CRISPR/Cas9 KO Plasmid (h) | sc-401298 | 20 µg | $397.00 |
Overview
AQP9 encodes aquaporin 9, an aquaglyceroporin plasma membrane channel that facilitates bidirectional transport of water and small solutes such as glycerol, urea, and other neutral metabolites. By regulating glycerol flux, AQP9 influences cellular energy balance and lipid metabolism, supporting processes including gluconeogenesis and osmotic homeostasis in metabolically active tissues. In immune and epithelial contexts, altered AQP9 activity has been linked to changes in inflammatory cell function and barrier physiology, connecting its expression to pathways that couple membrane transport with metabolic and stress responses. Dysregulated AQP9 expression has been reported across multiple disease-relevant settings, including metabolic disorders and cancer-associated phenotypes, where solute transport can shape proliferation and survival programs.
Aquaporin 9/AQP9 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the AQP9 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the AQP9 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the AQP9 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Aquaporin 9/AQP9 protein expression.
This CRISPR knockout system enables efficient generation of AQP9-deficient cell models for investigation of Aquaporin 9/AQP9 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting AQP9 exon(s) critical for Aquaporin 9/AQP9 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple AQP9 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Aquaporin 9/AQP9 CRISPR/Cas9 KO Plasmid (h) and Aquaporin 9/AQP9 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the AQP9 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Aquaporin 9/AQP9 HDR Plasmid (h) and Aquaporin 9/AQP9 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by AQP9 homology arms to support homology-directed repair at defined AQP9 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.