Aquaporin 5/AQP5 CRISPR/Cas9 KO Plasmid (m): sc-419176

Overview

Aqp5 encodes Aquaporin 5 (AQP5), a membrane water channel that supports rapid, osmotic water movement across epithelial barriers in secretory tissues such as salivary and lacrimal glands and the airway. By regulating transepithelial fluid transport and contributing to epithelial cell volume homeostasis, AQP5 influences processes including airway surface hydration, glandular secretion, and barrier function. Altered AQP5 expression or localization has been linked to dysregulated fluid handling and epithelial remodeling in inflammatory airway disease, exocrine gland dysfunction, and edema-associated tissue responses. In mouse systems, Aqp5 is frequently studied in the context of epithelial differentiation, ion and water transport coupling, and stress-responsive signaling that shapes mucosal physiology.

Aquaporin 5/AQP5 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Aqp5 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Aqp5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Aqp5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Aquaporin 5/AQP5 protein expression.

This CRISPR knockout system enables efficient generation of Aqp5-deficient cell models for investigation of Aquaporin 5/AQP5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Aqp5 exon(s) critical for Aquaporin 5/AQP5 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Aqp5 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Aquaporin 5/AQP5 CRISPR/Cas9 KO Plasmid (m) and Aquaporin 5/AQP5 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Aqp5 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Aquaporin 5/AQP5 HDR Plasmid (m) and Aquaporin 5/AQP5 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Aqp5 homology arms to support homology-directed repair at defined Aqp5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.