Aprotinin (CAS 9087-70-1)

Usage :

STORAGE AND SOLUBILITY: Aprotinin is freely soluble in water (>10 mg/mL) and in aqueous buffers of low ionic strengths. Dilute solutions are generally less stable than concentrated ones. Aprotinin solutions are relatively stable, although solution stability is highly dependent on pH. Solutions can be stored at 4°C for up to one week. For long term storage; addition of a carrier protein (ex: 0.1% BSA) is recommended, store at -20°C. Avoid freeze-thaw cycles.



The Cys14-Cys38 disulfide bridge is readily split by reducing agents like b-mercaptoethanol. Due to its compact tertiary structure, aprotinin is relatively stable against denaturation due to high temperature, acids, alkalies, organic solvents or proteolytic degradation (only thermolysin has been found capable of degrading aprotinin after heating to 60-80°C). The high basicity of aprotinin causes it to adhere to commonly used dialysis tubing and even gel filtration matrices, but the use of acetylated materials and concentrated salt solutions minimizes the problem. Sterilization may be achieved by filtration through a 0.2 mm filter.

Appearance :

Lyophilized powder

Physical State :

Solid

Solubility :

Soluble in water (5 mg/ml) and in aqueous buffers of low ionic strengths..

Storage :

Store at 4° C

Ki Data :

Chymotrypsinoge pH 8.0: K_i= 9 nM (bovine); Elastase pH 8.0: K_i= 3.5 µM (human leukocytes); Kallikrein (pancreatic), pH 8.0: K_i= 1.0 nM; Kallikrein (tissue): K_i= 1 nM; Plasmin pH 7.8: K_i= 4.0 nM; Trypsin pH 8.0: K_i= 0.06 pM (bovine); Urokinase pH 8.8: K_i= 8.0 µM (human)

WGK Germany :

1

RTECS :

YN5080000

PubChem CID :

Merck Index :

14: 757

MDL Number :

MFCD00130541

EC Number :

232-994-9

SMILES :

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