Aprotinin is a single chain polypeptide (58 amino-acids) crosslinked by three disulfide bridges, showing competitive and reversible inhibiton of proteolytic and esterolytic activity. Aprotinin forms stable complexes and blocks the active sites of serine protease enzymes. Binding is reversible with most aprotinin-protease complexes dissociating at pH >10 or < 3 (optimum pH 5-7); effective concentration is equimolar with protease. Used as a proteolytic inhibitor in radioimmunoassays of polypeptide hormones.
1. Fritz, H., 1983. Arzneimittelforschung. 33: 479-494. PMID: 6191764 2. Hewlett, G. 1990. Biotechnology. 8: 565-6, 568. PMID: 1369990 3. Levy, J.H., et al. 2004. Orthopedics. 27: s653-s658. PMID: 15239552 4. Keesey, J. ed. 1987. Biochemica Information.1st Ed. Boehringer Mannheim Biochemicals. Indianapolis, IN. 1987. p. 111.
STORAGE AND SOLUBILITY: Aprotinin is freely soluble in water (>10 mg/mL) and in aqueous buffers of low ionic strengths. Dilute solutions are generally less stable than concentrated ones. Aprotinin solutions are relatively stable, although solution stability is highly dependent on pH. Solutions can be stored at 4°C for up to one week. For long term storage; addition of a carrier protein (ex: 0.1% BSA) is recommended, store at -20°C. Avoid freeze-thaw cycles.
The Cys14-Cys38 disulfide bridge is readily split by reducing agents like b-mercaptoethanol. Due to its compact tertiary structure, aprotinin is relatively stable against denaturation due to high temperature, acids, alkalies, organic solvents or proteolytic degradation (only thermolysin has been found capable of degrading aprotinin after heating to 60-80°C). The high basicity of aprotinin causes it to adhere to commonly used dialysis tubing and even gel filtration matrices, but the use of acetylated materials and concentrated salt solutions minimizes the problem. Sterilization may be achieved by filtration through a 0.2 mm filter.
Soluble in water (5 mg/ml) and in aqueous buffers of low ionic strengths..
How many mg of aprotinin is needed to convert to kiu?
Asked by: two2igm05
Thank you for your question. The activity of Aprotinin, sc-3595, is 6300 KIU/mg (on dry basis).
Answered by: Chemical Support 4
Date published: 2017-03-07
What is the shelf life of sc-3595? How long is it stable?
Asked by: Jz28sail
Thank you for your question. The shelf life of sc-3595, Aprotinin, is dependent on handling and storage. Solutions are less stable than the lyophilized powder, but Lyophilized Aprotinin, stored at 4°C, is stable at least one year from date of purchase. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Rated 5 out of
Good protease inhibitorIt works well for PCR purification! I am going to buy it again
Date published: 2016-11-29
Rated 5 out of
It has been reported in several publicationsIt has been reported in several publications that product sc-3595 has been used as a reversible serine protease inhibitor in their lysis buffer. -SCBT Publication Review
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
You may not log in without verifying your email address. Please check your email (including your spam email) for the Welcome message that was sent.
Create a new account
Email address already exists, please enter a new valid email address.
USE YOUR SOCIAL NETWORK
Create an account quickly and easily with your preferred social network account. You won't have to remember an extra name and password.
Creating an account with us makes your shopping experience much easier and faster. You can save favorites, save cart, check order status and speed through checkout with saved addresses, payment methods and more.
Already have an account?
Please check your email (including your spam email) for a Welcome message, and verify your email address to continue.