APPBP1 Antibody (C-2): sc-390002
- APPBP1 Antibody (C-2) is a mouse monoclonal IgG2a κ APPBP1 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 161-347 mapping within an internal region of APPBP1 of human origin
- APPBP1 Antibody (C-2) is recommended for detection of APPBP1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-APPBP1 Antibody (C-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for APPBP1 Antibody (C-2) for WB applications. These reagents are now offered in bundles with APPBP1 Antibody (C-2) (see ordering information below).
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APPBP1 Antibody (C-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects APPBP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). APPBP1 Antibody (C-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. APPBP1, also known as β-Amyloid precursor protein-binding protein 1, NAE1 (NEDD8-activating enzyme E1 regulatory subunit 1), HPP1, or ula-1, is a crucial member of the ubiquitin-activating E1 family. APPBP1 is predominantly expressed in fetal tissues and is found throughout the brain in adult tissues, highlighting its significance in neural function. APPBP1 is associated with the cell membrane and acts as the regulatory subunit in a heterodimer with UBA3, forming a complex that binds to and activates NEDD8, a ubiquitin-like protein that plays a vital role in signal transduction, cell proliferation, and development. The interaction between APPBP1 and UBA3 is essential for proper cell cycle progression, particularly at the S/M checkpoint, where APPBP1 inhibits entry into the S phase while promoting entry into the M phase. This regulatory function underscores APPBP1′s importance in maintaining cellular homeostasis and its potential implications in various cellular processes, making anti-APPBP1 antibody (C-2) an invaluable tool for researchers studying these pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
APPBP1 Antibody (C-2) References:
- Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer. | Bohnsack, RN. and Haas, AL. 2003. J Biol Chem. 278: 26823-30. PMID: 12740388
- The structure of the APPBP1-UBA3-NEDD8-ATP complex reveals the basis for selective ubiquitin-like protein activation by an E1. | Walden, H., et al. 2003. Mol Cell. 12: 1427-37. PMID: 14690597
- A unique E1-E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8. | Huang, DT., et al. 2004. Nat Struct Mol Biol. 11: 927-35. PMID: 15361859
- Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1. | Huang, DT., et al. 2005. Mol Cell. 17: 341-50. PMID: 15694336
- Structural analysis of Escherichia coli ThiF. | Duda, DM., et al. 2005. J Mol Biol. 349: 774-86. PMID: 15896804
- Crystal structure of the interferon-induced ubiquitin-like protein ISG15. | Narasimhan, J., et al. 2005. J Biol Chem. 280: 27356-65. PMID: 15917233
- Expression, purification, and characterization of the E1 for human NEDD8, the heterodimeric APPBP1-UBA3 complex. | Huang, DT. and Schulman, BA. 2005. Methods Enzymol. 398: 9-20. PMID: 16275315
- Basis for a ubiquitin-like protein thioester switch toggling E1-E2 affinity. | Huang, DT., et al. 2007. Nature. 445: 394-8. PMID: 17220875
- APP-BP1 inhibits Abeta42 levels by interacting with Presenilin-1. | Chen, Y., et al. 2007. Mol Neurodegener. 2: 3. PMID: 17286867
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
APPBP1 Antibody (C-2) | sc-390002 | 200 µg/ml | $322.00 | |||
APPBP1 Antibody (C-2): m-IgGκ BP-HRP Bundle | sc-523366 | 200 µg Ab, 40 µg BP | $361.00 | |||
APPBP1 Antibody (C-2): m-IgG2a BP-HRP Bundle | sc-546158 | 200 µg Ab; 10 µg BP | $361.00 | |||
APPBP1 Antibody (C-2) AC | sc-390002 AC | 500 µg/ml, 25% agarose | $424.00 | |||
APPBP1 Antibody (C-2) HRP | sc-390002 HRP | 200 µg/ml | $322.00 | |||
APPBP1 Antibody (C-2) FITC | sc-390002 FITC | 200 µg/ml | $336.00 | |||
APPBP1 Antibody (C-2) PE | sc-390002 PE | 200 µg/ml | $349.00 | |||
APPBP1 Antibody (C-2) Alexa Fluor® 488 | sc-390002 AF488 | 200 µg/ml | $364.00 | |||
APPBP1 Antibody (C-2) Alexa Fluor® 546 | sc-390002 AF546 | 200 µg/ml | $364.00 | |||
APPBP1 Antibody (C-2) Alexa Fluor® 594 | sc-390002 AF594 | 200 µg/ml | $364.00 | |||
APPBP1 Antibody (C-2) Alexa Fluor® 647 | sc-390002 AF647 | 200 µg/ml | $364.00 | |||
APPBP1 Antibody (C-2) Alexa Fluor® 680 | sc-390002 AF680 | 200 µg/ml | $364.00 | |||
APPBP1 Antibody (C-2) Alexa Fluor® 790 | sc-390002 AF790 | 200 µg/ml | $364.00 |