apoO Double Nickase Plasmid (h): sc-413137-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- apoO Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- apoO Double Nickase Plasmid (h) and apoO Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting APOO. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
apoO Double Nickase Plasmid (h) | sc-413137-NIC | 20 µg | $410.00 | |||
apoO Double Nickase Plasmid (h2) | sc-413137-NIC-2 | 20 µg | $410.00 |
Human APOO encodes apolipoprotein O (apoO), a mitochondrial-associated apolipoprotein implicated in the regulation of oxidative phosphorylation and cellular energy metabolism. apoO has been linked to mitochondrial membrane organization and redox homeostasis, connecting it to pathways governing lipid handling, reactive oxygen species balance, and stress-responsive signaling. Altered APOO expression has been reported in contexts of metabolic dysfunction and cardiovascular-related phenotypes, consistent with a role in mitochondrial performance and lipid-associated processes. These features make APOO a useful target for dissecting how mitochondrial lipid-protein interactions influence bioenergetic state and downstream transcriptional programs.
apoO Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the APOO locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within APOO. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt APOO function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of APOO-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.