Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
apolipoprotein E/apoE CRISPR/Cas9 KO Plasmid (m): sc-419167
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- apolipoprotein E/apoE CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the apolipoprotein E/apoE genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: apolipoprotein E/apoE Antibody (F-9): sc-390925
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
apolipoprotein E/apoE CRISPR/Cas9 KO Plasmid (m) | sc-419167 | 20 µg | $397.00 |
Overview
Mouse Apoe encodes apolipoprotein E (apoE), a secreted and lipoprotein-associated protein that governs cholesterol and triglyceride transport in plasma and the central nervous system. ApoE mediates receptor-dependent uptake and redistribution of lipids through interactions with LDL receptor family members, shaping lipoprotein particle clearance, membrane homeostasis, and cellular cholesterol efflux pathways. In the brain, apoE participates in glial lipid trafficking, synaptic maintenance, and neuroinflammatory signaling, linking lipid metabolism to neuronal resilience. Altered Apoe function is widely used to model dyslipidemia and atherosclerosis mechanisms, and it is also central to experimental studies of amyloid deposition, microglial responses, and neurodegenerative processes.
apolipoprotein E/apoE CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Apoe gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Apoe together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Apoe open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish apolipoprotein E/apoE protein expression.
This CRISPR knockout system enables efficient generation of Apoe-deficient cell models for investigation of apolipoprotein E/apoE signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Apoe exon(s) critical for apolipoprotein E/apoE function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Apoe genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by apolipoprotein E/apoE CRISPR/Cas9 KO Plasmid (m) and apolipoprotein E/apoE CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Apoe locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by apolipoprotein E/apoE HDR Plasmid (m) and apolipoprotein E/apoE HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Apoe homology arms to support homology-directed repair at defined Apoe target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.