Date published: 2025-11-24

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apoL3 Antibody (L07): sc-101262

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Datasheets
  • apoL3 Antibody (L07) is a mouse monoclonal IgG2a κ apoL3 antibody provided at 50 µg/0.5 ml
  • raised against recombinant apoL3 of human origin
  • recommended for detection of apoL3 of human origin by WB, IP and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for apoL3 Antibody (L07). This work is in progress.

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    apoL3 Antibody (L07) is a mouse monoclonal IgG2a kappa light chain antibody that detects the apoL3 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). anti-apoL3 antibody (L07) is available as the non-conjugated isotype antibody. apoL3 (L07) monoclonal antibody recognizes a member of the apolipoprotein L gene family, which consists of six highly homologous proteins associated with high-density lipoproteins (HDL). These proteins play a crucial role in lipid metabolism and transport, influencing cellular lipid homeostasis and potentially impacting various physiological processes. Specifically, apoL3 localizes primarily in the cytoplasm and is widely expressed in several human tissues, with the highest expression levels found in the prostate, lung, and placenta. apoL3′s presence in these tissues suggests a significant role in lipid transport and metabolism, which is vital for maintaining cellular function and overall health. Additionally, apoL3 has been implicated in the movement of lipids within the cytoplasm, which is essential for cellular energy production and membrane synthesis. The human apoL family is mapped to chromosome 22q12.1-13.1, highlighting its genetic significance. apoL3 (L07) antibody serves as a valuable tool for researchers studying lipid metabolism and related disorders.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    apoL3 Antibody (L07) References:

    1. Vascular endothelial genes that are responsive to tumor necrosis factor-alpha in vitro are expressed in atherosclerotic lesions, including inhibitor of apoptosis protein-1, stannin, and two novel genes.  |  Horrevoets, AJ., et al. 1999. Blood. 93: 3418-31. PMID: 10233894
    2. Apolipoprotein L gene family: tissue-specific expression, splicing, promoter regions; discovery of a new gene.  |  Duchateau, PN., et al. 2001. J Lipid Res. 42: 620-30. PMID: 11290834
    3. The human apolipoprotein L gene cluster: identification, classification, and sites of distribution.  |  Page, NM., et al. 2001. Genomics. 74: 71-8. PMID: 11374903
    4. A genome annotation-driven approach to cloning the human ORFeome.  |  Collins, JE., et al. 2004. Genome Biol. 5: R84. PMID: 15461802
    5. Lipoprotein particles are required for Hedgehog and Wingless signalling.  |  Panáková, D., et al. 2005. Nature. 435: 58-65. PMID: 15875013
    6. APOL1 C-Terminal Variants May Trigger Kidney Disease through Interference with APOL3 Control of Actomyosin.  |  Uzureau, S., et al. 2020. Cell Rep. 30: 3821-3836.e13. PMID: 32187552
    7. Apolipoprotein L3 enhances CD8+ T cell antitumor immunity of colorectal cancer by promoting LDHA-mediated ferroptosis.  |  Lv, Y., et al. 2023. Int J Biol Sci. 19: 1284-1298. PMID: 36923931
    8. APOL3-LDHA axis related immunity activation and cancer ferroptosis induction.  |  Feng, Y. and Dai, Y. 2023. Int J Biol Sci. 19: 1401-1402. PMID: 37056935
    9. Intratumor APOL3 delineates a distinctive immunogenic ferroptosis subset with prognosis prediction in colorectal cancer.  |  Lv, Y., et al. 2024. Cancer Sci. 115: 257-269. PMID: 37986654
    10. Apolipoproteins L1 and L3 control mitochondrial membrane dynamics.  |  Lecordier, L., et al. 2023. Cell Rep. 42: 113528. PMID: 38041817
    11. Protein-truncating variant in APOL3 increases chronic kidney disease risk in epistasis with APOL1 risk alleles.  |  Zhang, DY., et al. 2024. JCI Insight. 9: PMID: 39163132
    12. Swimming upregulates APOL3 through regulating macrophage polarization to inhibit glycolysis and the development of melanoma.  |  Lyu, Z., et al. 2024. 3 Biotech. 14: 307. PMID: 39583207

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    apoL3 Antibody (L07)

    sc-101262
    50 µg/0.5 ml
    $316.00

    What are the recommended dilutions to use for WB and IP?

    Asked by: RK19
    Thank you for your question. apoL3 (L07) is recommended for detection of apoL3 of human origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunoprecipitation [1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)] and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000).
    Answered by: Tech Support Europe
    Date published: 2024-07-24

    For Western Blot, is it recommended to use denatured or non-denatured conditions with apoL3 (L07): sc-101262 antibody?

    Asked by: Germaine
    Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
    Answered by: Technical Support
    Date published: 2017-03-27
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    Rated 3 out of 5 by from Light band observed by Western Blot usingLight band observed by Western Blot using Jurkat whole cell lysate. Clean blot otherwise. No background. -SCBT QC
    Date published: 2014-06-19
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    apoL3 Antibody (L07) is rated 3.0 out of 5 by 1.
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