Date published: 2025-11-24

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Apolipoprotein A I/APOA1 Antibody (4): sc-135837

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Datasheets
  • Apolipoprotein A I/APOA1 Antibody (4) is a mouse monoclonal IgG1, cited in 1 publications, provided at 50 µg/500 µl
  • raised against amino acids 144-258 of apoA-I of rat origin
  • recommended for detection of apoA-I of mouse and rat origin by WB and IP
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Apolipoprotein A I/APOA1 Antibody (4). This work is in progress.

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apoA-I Antibody (4) is a mouse monoclonal IgG1 antibody that detects apolipoprotein A-I (apoA-I) in mouse and rat samples through applications such as western blotting (WB) and immunoprecipitation (IP). apoA-I is a crucial component of high-density lipoprotein (HDL) in plasma, playing a vital role in lipid metabolism by promoting the efflux of cholesterol from peripheral tissues to the liver for excretion. This process is essential for maintaining cholesterol homeostasis and preventing atherosclerosis, a condition characterized by the buildup of fats, cholesterol, and other substances in and on the artery walls. The structure of apoA-I, which consists of a single chain of 243 amino acids, allows apoA-I to interact effectively with lipids and other proteins, facilitating apoA-I′s function as a cofactor for lecithin cholesterol acyltransferase (LCAT). LCAT is responsible for the formation of most plasma cholesteryl esters, further underscoring apoA-I′s importance in lipid transport and metabolism. anti-apoA-I antibody (4) enables researchers to gain insights into apoA-I′s role in various physiological and pathological processes, enhancing our understanding of cardiovascular health and disease.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Apolipoprotein A I/APOA1 Antibody (4)

sc-135837
50 µg/500 µl
$316.00

I am using apoA-I (4): sc-135837 antibody for immunoprecipitation, how much antibody should I use?

Asked by: jenniferc
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Good specificity for WB in rat liver tissueGood specificity for WB in rat liver tissue extract. -SCBT QC
Date published: 2013-03-22
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Apolipoprotein A I/APOA1 Antibody (4) is rated 4.0 out of 5 by 1.
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