APLP1 Double Nickase Plasmid (h): sc-403937-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- APLP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- APLP1 Double Nickase Plasmid (h) and APLP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting APLP1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
APLP1 Double Nickase Plasmid (h) | sc-403937-NIC | 20 µg | $410.00 | |||
APLP1 Double Nickase Plasmid (h2) | sc-403937-NIC-2 | 20 µg | $410.00 |
APLP1 (amyloid beta precursor-like protein 1) is a neuronal type I transmembrane glycoprotein in the APP family that participates in synapse organization, neurite outgrowth, and activity-dependent signaling. It undergoes regulated proteolytic processing and contributes to cell–cell adhesion and vesicular trafficking processes that shape neuronal connectivity. APLP1 function intersects with pathways governing synaptic maturation, endocytic sorting, and membrane protein turnover, linking it to mechanisms that influence amyloidogenic processing networks in the nervous system. Altered APP-family biology and proteostasis have been associated with neurodegenerative disease-relevant phenotypes, making APLP1 a useful target for studying neuronal homeostasis and synaptic dysfunction.
APLP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the APLP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within APLP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt APLP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of APLP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.