Apelin Double Nickase Plasmid (h): sc-401548-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Apelin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Apelin Double Nickase Plasmid (h) and Apelin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting APLN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Apelin Antibody (2A1-2D5): sc-293441
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Apelin Double Nickase Plasmid (h) | sc-401548-NIC | 20 µg | $410.00 | |||
Apelin Double Nickase Plasmid (h2) | sc-401548-NIC-2 | 20 µg | $410.00 |
Human APLN encodes apelin, a secreted peptide ligand for the APJ/APLNR G protein–coupled receptor that regulates vascular tone, angiogenic signaling, cardiac contractility, and fluid homeostasis. Apelin–APJ engagement activates downstream pathways including PI3K/AKT, ERK/MAPK, and eNOS/NO signaling, shaping endothelial cell migration, smooth muscle responsiveness, and metabolic crosstalk in adipose and skeletal muscle. Dysregulated apelin signaling has been associated with cardiovascular remodeling, pulmonary vascular disease, and metabolic dysfunction, making APLN a useful node for studying peptide–GPCR communication in stress and inflammation. In tumor biology, apelin can influence hypoxia-adaptive programs and vascular niche dynamics, supporting mechanistic studies of microenvironmental signaling without implying clinical benefit.
Apelin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the APLN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within APLN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt APLN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of APLN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.