Apaf-1 Antibody (5E11): sc-65891

Apaf-1 Antibody (5E11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Apaf-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Apaf-1 antibody (5E11) is available in a non-conjugated form, allowing for versatile experimental use. Apaf-1, or apoptotic protease activating factor 1, plays a crucial role in the intrinsic pathway of apoptosis, acting as a key regulator in the activation of caspase-9, which subsequently leads to the execution of programmed cell death. This process is vital for maintaining cellular homeostasis and eliminating damaged or unwanted cells, thereby preventing the development of various diseases, including cancer. Apaf-1 is primarily located in the cytosol, where oligomerization occurs in response to apoptotic signals, forming a complex that recruits and activates caspase-9. Proper functioning of Apaf-1 is essential for the apoptotic cascade, and dysregulation of this pathway can lead to pathological conditions. Apaf-1 monoclonal antibody (5E11) is an invaluable tool for researchers studying apoptosis and its implications in health and disease, providing insights into the molecular mechanisms that govern cell death and survival.

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Apaf-1 Antibody (5E11) References:

1. Human CARD4 protein is a novel CED-4/Apaf-1 cell death family member that activates NF-kappaB.  |  Bertin, J., et al. 1999. J Biol Chem. 274: 12955-8. PMID: 10224040
2. Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-kappaB.  |  Inohara, N., et al. 1999. J Biol Chem. 274: 14560-7. PMID: 10329646
3. An induced proximity model for NF-kappa B activation in the Nod1/RICK and RIP signaling pathways.  |  Inohara, N., et al. 2000. J Biol Chem. 275: 27823-31. PMID: 10880512
4. Human Nod1 confers responsiveness to bacterial lipopolysaccharides.  |  Inohara, N., et al. 2001. J Biol Chem. 276: 2551-4. PMID: 11058605
5. Molecular cloning and characterization of DEFCAP-L and -S, two isoforms of a novel member of the mammalian Ced-4 family of apoptosis proteins.  |  Hlaing, T., et al. 2001. J Biol Chem. 276: 9230-8. PMID: 11076957
6. Is Apaf-1 expression frequently abrogated in melanoma?  |  Allen, JD., et al. 2005. Cell Death Differ. 12: 680-1. PMID: 15832174
7. Expression and function of the apoptosis effector Apaf-1 in melanoma.  |  Peltenburg, LT., et al. 2005. Cell Death Differ. 12: 678-9. PMID: 15832175
8. Expression of Apaf-1 in cervical cancer correlates with lymph node metastasis but not with intratumoral hypoxia.  |  Leo, C., et al. 2005. Gynecol Oncol. 97: 602-6. PMID: 15863166
9. Apaf-1: Regulation and function in cell death.  |  Shakeri, R., et al. 2017. Biochimie. 135: 111-125. PMID: 28192157
10. Apaf-1 Pyroptosome Senses Mitochondrial Permeability Transition.  |  Xu, W., et al. 2021. Cell Metab. 33: 424-436.e10. PMID: 33308446
11. Contribution of Apaf-1 to the pathogenesis of cancer and neurodegenerative diseases.  |  Shakeri, R., et al. 2021. Biochimie. 190: 91-110. PMID: 34298080
12. Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-kappaB.  |  Ogura, Y., et al. 2001. J Biol Chem. 276: 4812-8. PMID: 11087742

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Apaf-1 Antibody (5E11) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-Apaf-1 Antibody (5E11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Apaf-1 Antibody (5E11) Specifications

Alternate Product Name: Apaf-1

Product Clone Name: 5E11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Apaf1

Epitope Species: human

Product Reactivity/Detection: Apaf-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 50 µg IgG₁ kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for Apaf-1 Antibody (5E11): N/A