AP-4 Antibody (H-5): sc-166216

AP-4 Antibody (H-5) is a mouse monoclonal IgG1 antibody that detects AP-4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AP-4, a member of the AP transcription factor family, plays a crucial role in gene regulation by specifically binding to the DNA consensus sequence 5′-CAGCTG-3′, which is vital for the transcription of various genes, including those involved in immune response and cellular growth. AP-4′s ability to interact with promoters for immunoglobulin-κ gene families and simian virus 40 highlights AP-4′s importance in both normal cellular functions and disease states, such as cancer. Structurally, AP-4 is characterized by a helix-loop-helix configuration, which is essential for DNA-binding activity and interaction with other transcription factors. Additionally, AP-4 contains two distinctive leucine repeat elements that contribute to stability and functional versatility, making AP-4 a significant player in the transcriptional regulation of genes associated with critical biological processes. The gene encoding AP-4 is located on human chromosome 12q24, and AP-4 expression is tightly regulated, underscoring AP-4′s importance in maintaining cellular homeostasis and responding to external stimuli.

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AP-4 Antibody (H-5) References:

1. AP-4, a novel protein complex related to clathrin adaptors.  |  Dell'Angelica, EC., et al. 1999. J Biol Chem. 274: 7278-85. PMID: 10066790
2. AP-4 binds basolateral signals and participates in basolateral sorting in epithelial MDCK cells.  |  Simmen, T., et al. 2002. Nat Cell Biol. 4: 154-9. PMID: 11802162
3. AP‑4 predicts poor prognosis in non‑small cell lung cancer.  |  Gong, H., et al. 2014. Mol Med Rep. 10: 336-40. PMID: 24804973
4. The Adaptor Complex AP-4 Regulates Vacuolar Protein Sorting at the trans-Golgi Network by Interacting with VACUOLAR SORTING RECEPTOR1.  |  Fuji, K., et al. 2016. Plant Physiol. 170: 211-9. PMID: 26546666
5. Silencing of AP-4 inhibits proliferation, induces cell cycle arrest and promotes apoptosis in human lung cancer cells.  |  Hu, X., et al. 2016. Oncol Lett. 11: 3735-3742. PMID: 27313685
6. Axonal autophagosome maturation defect through failure of ATG9A sorting underpins pathology in AP-4 deficiency syndrome.  |  Ivankovic, D., et al. 2020. Autophagy. 16: 391-407. PMID: 31142229
7. Second messenger Ap4A polymerizes target protein HINT1 to transduce signals in FcεRI-activated mast cells.  |  Yu, J., et al. 2019. Nat Commun. 10: 4664. PMID: 31604935
8. The FTS-Hook-FHIP (FHF) complex interacts with AP-4 to mediate perinuclear distribution of AP-4 and its cargo ATG9A.  |  Mattera, R., et al. 2020. Mol Biol Cell. 31: 963-979. PMID: 32073997
9. The Reelin receptor ApoER2 is a cargo for the adaptor protein complex AP-4: Implications for Hereditary Spastic Paraplegia.  |  Caracci, MO., et al. 2024. Prog Neurobiol. 234: 102575. PMID: 38281682
10. Lymphocytic choriomeningitis arenavirus requires cellular COPI and AP-4 complexes for efficient virion production.  |  Byford, O., et al. 2024. J Virol. 98: e0200623. PMID: 38334330

The preferred AP-4 antibody is AP-4 Antibody (A-8): sc-377042.