AP-4 Antibody (F-1): sc-376977

AP-4 Antibody (F-1) is a mouse monoclonal IgG1 antibody that detects AP-4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AP-4, a member of the AP transcription factor family, plays a crucial role in gene regulation by specifically binding to the DNA consensus sequence 5′-CAGCTG-3′, which is vital for the transcription of various target genes, including those involved in immune response and cellular growth. AP-4 (F-1) antibody′s ability to detect interactions with promoters for immunoglobulin-κ gene families and simian virus 40 highlights AP-4′s importance in both normal cellular functions and disease states, such as cancer. Additionally, AP-4 is characterized by its helix-loop-helix structure, which is essential for DNA-binding capability and interaction with other transcription factors, thereby influencing the expression of genes critical for cellular processes. The gene encoding AP-4 is located on human chromosome 12q24, and AP-4 expression is tightly regulated, making AP-4 a significant focus for research in developmental biology and oncology. Anti-AP-4 antibody (F-1) is an invaluable tool for researchers aiming to explore the functional dynamics of AP-4 in various biological contexts.

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AP-4 Antibody (F-1) References:

1. Adaptor protein complex-4 (AP-4) is expressed in the central nervous system neurons and interacts with glutamate receptor delta2.  |  Yap, CC., et al. 2003. Mol Cell Neurosci. 24: 283-95. PMID: 14572453
2. Adaptor protein complex-4 (AP-4) deficiency causes a novel autosomal recessive cerebral palsy syndrome with microcephaly and intellectual disability.  |  Moreno-De-Luca, A., et al. 2011. J Med Genet. 48: 141-4. PMID: 20972249
3. Transcription factor AP-4 contains multiple dimerization domains that regulate dimer specificity.  |  Hu, YF., et al. 1990. Genes Dev. 4: 1741-52. PMID: 2123466
4. The epsilon hinge-ear region regulates membrane localization of the AP-4 complex.  |  Paolini, L., et al. 2011. Traffic. 12: 1604-19. PMID: 21810154
5. Down-regulation of AP-4 inhibits proliferation, induces cell cycle arrest and promotes apoptosis in human gastric cancer cells.  |  Liu, X., et al. 2012. PLoS One. 7: e37096. PMID: 22615908
6. Bivalent Motif-Ear Interactions Mediate the Association of the Accessory Protein Tepsin with the AP-4 Adaptor Complex.  |  Mattera, R., et al. 2015. J Biol Chem. 290: 30736-49. PMID: 26542808
7. Enhancer binding factors AP-4 and AP-1 act in concert to activate SV40 late transcription in vitro.  |  Mermod, N., et al. 1988. Nature. 332: 557-61. PMID: 2833704
8. Disease Severity and Motor Impairment Correlate With Health-Related Quality of Life in AP-4-Associated Hereditary Spastic Paraplegia.  |  Jordan, C., et al. 2021. Neurol Genet. 7: e605. PMID: 34295967
9. An Ultra-Rare Mixed Phenotype with Combined AP-4 and ERF Mutations: The First Report in a Pediatric Patient and a Literature Review.  |  Orsini, A., et al. 2024. Genes (Basel). 15: PMID: 38674371
10. Axonal organelle buildup from loss of AP-4 complex function causes exacerbation of amyloid plaque pathology and gliosis in Alzheimer's disease mouse model.  |  Orlowski, A., et al. 2024. eNeuro. 11: PMID: 39632089

The preferred AP-4 antibody is AP-4 Antibody (A-8): sc-377042.