AP-3β Antibody (3B4): sc-517083

AP-3β Antibody (3B4) is a mouse monoclonal IgG2a kappa light chain antibody that detects AP-3β in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-AP-3β antibody (3B4) is available in a non-conjugated form, allowing for versatile use in various experimental setups. AP-3β, also known as β3A-Adaptin, plays a crucial role in sorting proteins within exocytic and endocytic pathways, forming part of the adaptor-like complex AP-3, which consists of four distinct subunits. AP-3β is essential for proper trafficking of membrane proteins, particularly in neurons, where AP-3β is involved in synaptic vesicle transport. AP-3β shares significant structural similarity with β-NAP, a neuron-specific protein, exhibiting 61% overall identity. The interaction between AP-3β and β-NAP highlights functional redundancy and specialization of adaptor proteins in cellular transport mechanisms. AP-3β undergoes post-translational modifications, such as phosphorylation by casein kinase I at its hinge domain, which is critical for function; inhibiting this kinase can disrupt AP-3 recruitment to synaptic vesicles, thereby affecting neurotransmitter release and synaptic plasticity.

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AP-3β Antibody (3B4) References:

1. The AP-3 complex required for endosomal synaptic vesicle biogenesis is associated with a casein kinase Ialpha-like isoform.  |  Faundez, VV. and Kelly, RB. 2000. Mol Biol Cell. 11: 2591-604. PMID: 10930456
2. Distinct requirements for the AP-3 adaptor complex in pigment granule and synaptic vesicle biogenesis in Drosophila melanogaster.  |  Mullins, C., et al. 2000. Mol Gen Genet. 263: 1003-14. PMID: 10954086
3. A novel adaptor-related protein complex.  |  Simpson, F., et al. 1996. J Cell Biol. 133: 749-60. PMID: 8666661
4. AP-3: an adaptor-like protein complex with ubiquitous expression.  |  Dell'Angelica, EC., et al. 1997. EMBO J. 16: 917-28. PMID: 9118953
5. Characterization of the adaptor-related protein complex, AP-3.  |  Simpson, F., et al. 1997. J Cell Biol. 137: 835-45. PMID: 9151686
6. Beta3A-adaptin, a subunit of the adaptor-like complex AP-3.  |  Dell'Angelica, EC., et al. 1997. J Biol Chem. 272: 15078-84. PMID: 9182526
7. Association of the AP-3 adaptor complex with clathrin.  |  Dell'Angelica, EC., et al. 1998. Science. 280: 431-4. PMID: 9545220

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AP-3β Antibody (3B4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-AP-3β Antibody (3B4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AP-3β Antibody (3B4) Specifications

Alternate Product Name: AP-3β

Product Clone Name: 3B4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 995-1094

Epitope Target: AP-3β

Epitope Species: human

Product Reactivity/Detection: AP-3β

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for AP-3β Antibody (3B4): N/A