anti-Cy3 Antibody (A-6): sc-166894

Anti-Cy3 Antibody (A-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects Cy3 protein in non-species reactive applications through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Cy3 antibody (A-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Cy3 protein is part of the cyanine dye family, which is crucial in biomedical imaging due to its ability to emit fluorescence across a wide spectrum, from infrared to ultraviolet. This property allows researchers to visualize and track biological processes with high sensitivity and specificity. The structural characteristics of cyanine dyes, including their partially saturated indole nitrogen heterocyclic nucleus and the polyalkene bridge, contribute to unique optical properties, making these dyes invaluable tools in various applications, such as enhancing the sensitivity of photographic emulsions and facilitating advanced imaging techniques. Anti-Cy3 antibody (A-6) can be utilized in a linker system to bind cyanine dyes, enabling the development of activity-based probes that target specific enzymes at their active sites, thereby expanding the potential for innovative research applications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

anti-Cy3 Antibody (A-6) References:

1. Verification of specific G-quadruplex structure by using a novel cyanine dye supramolecular assembly: II. The binding characterization with specific intramolecular G-quadruplex and the recognizing mechanism.  |  Yang, Q., et al. 2010. Nucleic Acids Res. 38: 1022-33. PMID: 19933263
2. Synthesis and spectroscopic and DNA-binding properties of fluorogenic acridine-containing cyanine dyes.  |  Mahmood, T., et al. 2010. J Org Chem. 75: 204-7. PMID: 19954141
3. Fluorescence lifetime properties of near-infrared cyanine dyes in relation to their structures.  |  Lee, H., et al. 2008. J Photochem Photobiol A Chem. 200: 438-444. PMID: 20016664
4. Fast assembly of cyanine dyes into aggregates onto [6,6]-phenyl C61-butyric acid methyl ester surfaces from organic solvents.  |  Heier, J., et al. 2010. Langmuir. 26: 3955-61. PMID: 20073528
5. Electronic and vibrational coherence dynamics in a cyanine dye studied using a few-cycle pulsed laser.  |  Wang, Y. and Kobayashi, T. 2010. Chemphyschem. 11: 889-96. PMID: 20135666
6. Perylene-Cy3 FRET System to Analyze Photoactive DNA Structures.  |  Kawai, H., et al. 2021. Chemistry. 27: 12845-12850. PMID: 34269491
7. Cy3-RgIA-5727 Labels and Inhibits α9-Containing nAChRs of Cochlear Hair Cells.  |  Fisher, F., et al. 2021. Front Cell Neurosci. 15: 697560. PMID: 34385908
8. Cy3-ATP labeling of unfixed, permeabilized mouse hair cells.  |  Pacentine, IV. and Barr-Gillespie, PG. 2021. Sci Rep. 11: 23855. PMID: 34903829
9. Sequence-dependence of Cy3 and Cy5 dyes in 3' terminally-labeled single-stranded DNA.  |  Kekić, T. and Lietard, J. 2022. Sci Rep. 12: 14803. PMID: 36045146
10. Cy3 Cyanine Dye with Strong Fluorescence Enhancement for AGRO100 and Its Derivative.  |  Ma, X., et al. 2023. J Phys Chem B. 127: 1811-1818. PMID: 36802619
11. Detecting RNA-Protein Interactions With EGFP-Cy3 FRET by Acceptor Photobleaching.  |  Alam, SR., et al. 2023. Curr Protoc. 3: e689. PMID: 36821783
12. Evaluation of bioorthogonally applicable tetrazine-Cy3 probes for fluorogenic labeling schemes.  |  Albitz, E., et al. 2023. Org Biomol Chem. 21: 7358-7366. PMID: 37646224

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-anti-Cy3 Antibody (A-6) has 11 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

anti-Cy3 Antibody (A-6) Specifications

Alternate Product Name: anti-Cy3

Product Clone Name: A-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Cy3

Epitope Species: N/A

Product Reactivity/Detection: Cy3

Species Reactivity/Detection: NSR

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for anti-Cy3 Antibody (A-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin