Annexin XI Double Nickase Plasmid (h): sc-404879-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Annexin XI Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Annexin XI Double Nickase Plasmid (h) and Annexin XI Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ANXA11. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Annexin XI Antibody (D-12): sc-46686
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Annexin XI Double Nickase Plasmid (h) | sc-404879-NIC | 20 µg | $410.00 | |||
Annexin XI Double Nickase Plasmid (h2) | sc-404879-NIC-2 | 20 µg | $410.00 |
ANXA11 encodes annexin XI, a Ca2+-dependent phospholipid-binding protein that associates with intracellular membranes and participates in membrane trafficking, vesicle dynamics, and cytoskeletal organization. Annexin XI has been linked to endosomal and lysosomal processes, including roles in late endosome function and autophagy-related pathways, and its membrane-binding activity is modulated by calcium signaling. Cellular studies implicate ANXA11 in stress-responsive assemblies and RNA granule dynamics, connecting it to proteostasis and organelle homeostasis. Genetic and functional perturbations of ANXA11 have been associated with neurodegenerative and neuromuscular disease biology, making it relevant for mechanistic research on protein aggregation, axonal maintenance, and cellular stress responses.
Annexin XI Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ANXA11 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ANXA11. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ANXA11 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ANXA11-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.