Annexin-2/ANXA2 Double Nickase Plasmid (h): sc-400296-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Annexin-2/ANXA2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Annexin-2/ANXA2 Double Nickase Plasmid (h) and Annexin-2/ANXA2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ANXA2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Annexin-2/ANXA2 Antibody (C-10): sc-28385
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Annexin-2/ANXA2 Double Nickase Plasmid (h) | sc-400296-NIC | 20 µg | $410.00 | |||
Annexin-2/ANXA2 Double Nickase Plasmid (h2) | sc-400296-NIC-2 | 20 µg | $410.00 |
ANXA2 encodes annexin A2, a Ca²⁺-dependent phospholipid-binding protein that associates with the plasma membrane and cortical actin to coordinate membrane trafficking, cytoskeletal remodeling, and cell motility. Annexin A2 functions in complexes with S100A10 to support plasmin generation and pericellular proteolysis, linking it to extracellular matrix turnover and wound-associated signaling. It contributes to endocytosis/exocytosis, lipid raft organization, and regulation of fibrinolytic and inflammatory pathways. Dysregulated ANXA2 expression or localization has been reported across multiple tumor types and vascular/inflammatory conditions, making it a useful node for studying invasion, angiogenic responses, and stress-adaptive membrane dynamics.
Annexin-2/ANXA2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ANXA2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ANXA2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ANXA2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ANXA2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.