ANKRD37 Double Nickase Plasmid (h): sc-410438-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ANKRD37 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ANKRD37 Double Nickase Plasmid (h) and ANKRD37 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ANKRD37. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ANKRD37 Double Nickase Plasmid (h) | sc-410438-NIC | 20 µg | $410.00 |
ANKRD37 (ankyrin repeat domain 37) encodes a hypoxia-responsive protein whose expression is strongly regulated by HIF signaling, linking oxygen sensing to transcriptional adaptation. It is commonly used as a molecular readout of HIF-1 pathway activity and participates in cellular programs that remodel metabolism, survival, and stress responses under low-oxygen conditions. Altered ANKRD37 expression has been reported across hypoxia-enriched microenvironments, including solid tumor biology and ischemic or inflammatory settings, where it can reflect shifts in oxygen-dependent gene regulatory networks. These features make ANKRD37 relevant for studying hypoxia-driven transcriptional programs, pathway crosstalk, and context-dependent phenotypes in human cells.
ANKRD37 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ANKRD37 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ANKRD37. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ANKRD37 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ANKRD37-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.