ANKRD22 Double Nickase Plasmid (h): sc-416874-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ANKRD22 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ANKRD22 Double Nickase Plasmid (h) and ANKRD22 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ANKRD22. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ANKRD22 Double Nickase Plasmid (h) | sc-416874-NIC | 20 µg | $410.00 | |||
ANKRD22 Double Nickase Plasmid (h2) | sc-416874-NIC-2 | 20 µg | $410.00 |
ANKRD22 encodes an ankyrin repeat–containing protein implicated in protein–protein interactions that influence cellular differentiation and stress-responsive transcriptional programs. Reported expression patterns link ANKRD22 to epithelial biology and metabolic adaptation, with suggested roles in regulating proliferation and survival pathways under altered nutrient or oxygen conditions. Dysregulated ANKRD22 expression has been associated with tumor biology in multiple datasets, supporting its use as a molecular node for investigating oncogenic signaling, cell state transitions, and context-dependent gene regulation. Studying ANKRD22 function can help clarify how ankyrin repeat scaffolding proteins interface with pathway dynamics that shape growth, migration, and cellular homeostasis.
ANKRD22 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ANKRD22 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ANKRD22. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ANKRD22 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ANKRD22-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.