Date published: 2025-12-3

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Aniline Blue W/S certified (CAS 28631-66-5)

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Alternate Names:
Acid Blue 22
Application:
Aniline Blue W/S certified is a biological stain used for staining collagen
CAS Number:
28631-66-5
Molecular Weight:
737.72
Molecular Formula:
C32H25N3O9S3Na2
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Aniline Blue W/S certified is a water-soluble dye that belongs to the family of triphenylmethane dyes, known for its intense blue color. This dye specifically binds to acidic components of cells and tissues, such as nucleic acids and polysaccharides, making it an invaluable tool for visualizing complex structures within biological specimens. One of the primary applications of Aniline Blue W/S in biological staining is in the visualization of plant cell walls. By staining these structures, researchers can study the cell wall composition, structure, and changes during growth, development, or in response to environmental stress. This information is for understanding plant physiology and pathology, as well as for agricultural research aimed at improving crop resilience and productivity. In addition to plant studies, Aniline Blue W/S is used in the Masson′s trichrome staining technique, a method employed to distinguish between muscle, collagen, and cytoplasm in tissue sections. The blue stain specifically highlights collagen fibers in tissue samples, allowing for detailed analysis of connective tissue structure and identification of pathological changes


Aniline Blue W/S certified (CAS 28631-66-5) References

  1. Addition of eosin to the aniline blue assay to enhance detection of immature sperm histones.  |  Wong, A., et al. 2008. Fertil Steril. 90: 1999-2002. PMID: 18177870
  2. Equilibrium, kinetic and sorber design studies on the adsorption of Aniline blue dye by sodium tetraborate-modified Kaolinite clay adsorbent.  |  Unuabonah, EI., et al. 2008. J Hazard Mater. 157: 397-409. PMID: 18343030
  3. In vivo toxicity testing of methyl blue and aniline blue as vital dyes for intraocular surgery.  |  Thaler, S., et al. 2009. Retina. 29: 1257-65. PMID: 19934820
  4. Experimental evaluation of aniline and methyl blue for intraocular surgery.  |  Haritoglou, C., et al. 2009. Retina. 29: 1266-73. PMID: 19934821
  5. Decolorization and detoxification of a sulfonated triphenylmethane dye aniline blue by Shewanella oneidensis MR-1 under anaerobic conditions.  |  Wu, Y., et al. 2013. Appl Microbiol Biotechnol. 97: 7439-46. PMID: 23053116
  6. Modeling studies: Adsorption of aniline blue by using Prosopis Juliflora carbon/Ca/alginate polymer composite beads.  |  Kumar, M. and Tamilarasan, R. 2013. Carbohydr Polym. 92: 2171-80. PMID: 23399273
  7. The utility of sperm DNA damage assay using toluidine blue and aniline blue staining in routine semen analysis.  |  Kim, HS., et al. 2013. Clin Exp Reprod Med. 40: 23-8. PMID: 23614112
  8. Fast Chromotrope Aniline Blue Special Stain Is a Useful Tool to Assess Fibrosis on Liver Biopsy During Transplantation.  |  Cima, L., et al. 2017. Transplant Proc. 49: 667-670. PMID: 28457367
  9. Evaluation of Sperm Chromatin Integrity Using Aniline Blue and Toluidine Blue Staining in Infertile and Normozoospermic Men.  |  Pourmasumi, S., et al. 2019. J Reprod Infertil. 20: 95-101. PMID: 31058054
  10. Rapid screening method of Saccharomyces cerevisiae mutants using calcofluor white and aniline blue.  |  Perrine-Walker, F. and Payne, J. 2021. Braz J Microbiol. 52: 1077-1086. PMID: 33948877
  11. Tunable Cytotoxicity and Selectivity of Phosphonium Ionic Liquid with Aniline Blue Dye.  |  Macchi, S., et al. 2021. J Nanosci Nanotechnol. 21: 6143-6150. PMID: 34229815
  12. Novosphingobium decolorationis sp. nov., an aniline blue-decolourizing bacterium isolated from East Pacific sediment.  |  Chen, X., et al. 2021. Int J Syst Evol Microbiol. 71: PMID: 34524955
  13. Efficient photocatalytic degradation of aniline blue under solar irradiation by ternary cobalt ferrite/graphitic carbon nitride/bentonite nanocomposite.  |  Thakurata, DG., et al. 2022. Environ Sci Pollut Res Int. 29: 34269-34277. PMID: 35037149
  14. Facile fabrication of a novel BiPO4 phase junction with enhanced photocatalytic performance towards aniline blue degradation.  |  Azzam, AB., et al. 2019. RSC Adv. 9: 17246-17253. PMID: 35519846
  15. Aniline blue test for sperm protamination-a valuable add-on to the bull breeding soundness evaluation.  |  Alfadel, F., et al. 2023. Trop Anim Health Prod. 55: 76. PMID: 36764981

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Aniline Blue W/S certified, 25 g

sc-291900
25 g
$83.00

Aniline Blue W/S certified, 100 g

sc-291900A
100 g
$236.00

Aniline Blue W/S certified, 250 g

sc-291900B
250 g
$414.00

Aniline Blue W/S certified, 1 kg

sc-291900C
1 kg
$1169.00