Amylin Antibody (E-5) recommended for detection of Amylin precursor and active peptide of human origin by WB, IP, IF, IHC(P) and ELISA

Amylin Antibody (E-5): sc-377530

Amylin Antibody (E-5) is rated 4.6 out of 5 by 5.
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Datasheets

    • Anti-Amylin Antibody (E-5) is a mouse monoclonal IgG2a (kappa light chain) Amylin antibody provided at 200 µg/ml
    • raised against amino acids 40-89 mapping at the C-terminus of Amylin of human origin
    • Amylin Antibody (E-5) is recommended for detection of Amylin precursor and active peptide of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Amylin Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Amylin (E-5): sc-377530.

What do you mean by the antibody detecting "Amylin precursor and active peptide"? Does this refer to monomeric or oligomeric amylin?

Asked by: KaraZ
Thank you for your question. This monoclonal antibody is raised against amino acids 40-89 of the full length human protein, which, according to Uniprot is processed into a Islet amyloid polypeptide, corresponding to amino acids 34-70. Therefore, we expect the antibody to recognize both precursor and active processed forms of Amylin. https://www.uniprot.org/uniprot/P10997#ptm_processing
Answered by: Technical Support
Date published: 2019-09-27

How specific is the antibody for human amylin? Is there any cross reactivity for amylin of other species (especially rat)?

Asked by: Susan1
Thank you for your question. We have not yet completed testing of this antibody, Amylin (E-5): sc-377530, for reactivity in other species including mouse and rat. The antibody is raised against an antigen sequence derived from human Amylin, however that sequence does share ~ 70% sequence identity with the corresponding protein in mouse and rat. The exact epitope is not known for this antibody, but there is potential for cross-reactivity with these other species.
Answered by: Technical Support
Date published: 2017-11-02

Is Amylin Antibody (E-5): sc-377530 appropriate for use in IHC with paraffin-embedded sections?

Asked by: jenni
Thank you for your question. Yes, Amylin Antibody (E-5): sc-377530 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Service 11
Date published: 2017-01-09

Hi, l would like to use your Amylin antibody E-5 and H-50 as bioreceptors in my Biacore assay, to detect Amylin as analyte based on antigen-antibody binding. Could you please send me the links of Amylin that I should I buy? Thank you very much in advance!

Asked by: Evelyn
Thank you for your question. We have not tested any of our antibodies using the Biacore assay and so we are unable to offer you any specific support using the antibodies in this system. We do not currently sell any Amylin protein or peptide that could be used as a synthetic antigen with our Amylin antibodies.
Answered by: Technical Support
Date published: 2016-10-06
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Rated 5 out of 5 by from Good antibody Good, published Amylin antibody for human detection. Strong signal observed in WB and staining, and is also recommended for IP and ELISA.
Date published: 2017-01-20
Rated 5 out of 5 by from Superb product Outstanding IHC staining data; also reacts well for WB
Date published: 2017-01-09
Rated 4 out of 5 by from Worked well in our WB Worked well in our WB.
Date published: 2015-08-05
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmic Produced exceptional immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-03-10
Rated 4 out of 5 by from Robust signal in WB in human transfected Robust signal in WB in human transfected whole cell lysates. -SCBT QC
Date published: 2015-02-05
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