Amylin Antibody (E-5): sc-377530

Amylin Antibody (E-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects Amylin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Amylin antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Amylin, also known as Islet Amyloid Polypeptide (IAPP), is a 37-amino acid polypeptide that plays a crucial role in glucose metabolism and is secreted by pancreatic beta cells alongside insulin. Amylin′s primary function is to regulate glucose levels by slowing gastric emptying and promoting satiety, which is particularly important in the context of diabetes, as dysregulation of Amylin can contribute to the pathophysiology of type 2 diabetes. Amylin forms amyloid fibrils in the pancreas of diabetic patients, which can lead to beta-cell dysfunction and loss, highlighting Amylin′s significance in both normal physiology and disease states. The gene encoding Amylin is located on human chromosome 12p12.3-p12.1, and Amylin expression is tightly regulated, making Amylin a valuable target for research into metabolic disorders.

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Amylin Antibody (E-5) References:

1. Functional interaction of G protein-coupled receptors of the adrenomedullin peptide family with accessory receptor-activity-modifying proteins (RAMP).  |  Born, W., et al. 2002. Microsc Res Tech. 57: 14-22. PMID: 11921352
2. Amylin in human pancreatic islets.  |  Tomita, T. 2003. Pathology. 35: 34-6. PMID: 12701681
3. Fibrillogenic amylin evokes islet beta-cell apoptosis through linked activation of a caspase cascade and JNK1.  |  Zhang, S., et al. 2003. J Biol Chem. 278: 52810-9. PMID: 14532296
4. Human amylin oligomer growth and fibril elongation define two distinct phases in amyloid formation.  |  Green, JD., et al. 2004. J Biol Chem. 279: 12206-12. PMID: 14704152
5. Peripheral amylin activates circumventricular organs expressing calcitonin receptor a/b subtypes and receptor-activity modifying proteins in the rat.  |  Barth, SW., et al. 2004. Brain Res. 997: 97-102. PMID: 14715154
6. Amylin inhibits bone resorption while the calcitonin receptor controls bone formation in vivo.  |  Dacquin, R., et al. 2004. J Cell Biol. 164: 509-14. PMID: 14970190
7. The parallel superpleated beta-structure as a model for amyloid fibrils of human amylin.  |  Kajava, AV., et al. 2005. J Mol Biol. 348: 247-52. PMID: 15811365
8. Valsartan inhibits amylin-induced podocyte damage.  |  Huang, F., et al. 2016. Microvasc Res. 106: 101-9. PMID: 27102209
9. Purification and characterization of a peptide from amyloid-rich pancreases of type 2 diabetic patients.  |  Cooper, GJ., et al. 1987. Proc Natl Acad Sci U S A. 84: 8628-32. PMID: 3317417
10. Future targets for migraine treatment beyond CGRP.  |  Al-Hassany, L., et al. 2023. J Headache Pain. 24: 76. PMID: 37370051
11. Skin capillary amylin deposition resembles brain amylin vasculopathy in rats.  |  Das, S., et al. 2023. J Stroke Cerebrovasc Dis. 32: 107300. PMID: 37572602
12. High-resolution chromosomal localization of the human calcitonin/CGRP/IAPP gene family members.  |  Hoovers, JM., et al. 1993. Genomics. 15: 525-9. PMID: 8468047

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Amylin Antibody (E-5) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-Amylin Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Amylin Antibody (E-5) Specifications

Alternate Product Name: IAPP

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 40-89

Epitope Target: Amylin

Epitope Species: human

Product Reactivity/Detection: Amylin precursor and active peptide

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for Amylin Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin