Amphiphysin I Antibody (15): sc-135830

Amphiphysin I Antibody (15) is a mouse monoclonal IgG1 antibody that detects Amphiphysin I in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Amphiphysin I is a crucial brain-enriched protein that plays a significant role in synaptic vesicle recycling, a process vital for neurotransmission and overall brain function. Anti-Amphiphysin I antibody (15) recognizes a protein that functions as a dimer and features a unique structure including a membrane-bending BAR domain, a central Clathrin and adaptor binding domain, and a C-terminal SH3 domain. The interaction of Amphiphysin I with the GTPase Dynamin via its SH3 domain is essential for proper binding of Dynamin to Clathrin-coated pits, facilitating vesicle budding and release at synapses. In addition to its role in the brain, Amphiphysin I is also implicated in membrane bending and curvature stabilization in various tissues, highlighting its importance in cellular dynamics. Notably, the mammalian Amphiphysins, including Amphiphysin I and its splice variant Amphiphysin II, share a similar overall structure, yet the splice form of Amphiphysin II, which lacks Clathrin or adaptor interactions, is predominantly expressed in muscle tissue and is critical for the formation and stabilization of the T tubule network. Amphiphysin I monoclonal antibody (15) is an invaluable tool for researchers studying synaptic function and membrane dynamics across different biological systems.

The preferred Amphiphysin I antibody is Amphiphysin I Antibody (G-4): sc-376402.