Amnionless Antibody (F-7): sc-365734

Amnionless Antibody (F-7) is a mouse monoclonal IgG1 antibody that detects Amnionless in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Amnionless is a vital membrane protein encoded by the AMN gene, playing a crucial role in the absorption of vitamin B12, which is essential for DNA synthesis and cellular function. Defects in the AMN gene lead to megaloblastic anemia 1, a hereditary disorder characterized by selective malabsorption of vitamin B12, resulting in impaired thymidine synthase activity and disrupted DNA synthesis. Amnionless is primarily expressed in the colon, kidney, and small intestine, where Amnionless interacts with cubulin to facilitate vitamin B12 uptake. Additionally, shorter isoforms of Amnionless can be found in the thymus, testis, and peripheral blood leukocytes, highlighting diverse roles in various tissues. Understanding the function and significance of Amnionless is critical, as Amnionless not only impacts vitamin B12 absorption but also modulates bone morphogenetic protein signaling pathways, which are essential for trunk mesoderm production during embryonic development. Anti-Amnionless antibody (F-7) is an invaluable tool for researchers investigating the molecular mechanisms underlying vitamin B12 absorption and related disorders.

The preferred Amnionless antibody is Amnionless Antibody (C-10): sc-365384.