Ammonium phosphate monobasic solution (CAS 7722-76-1)

Ammonium phosphate monobasic solution, a clear or slightly cloudy liquid, is a vital chemical reagent with multifaceted applications in scientific research, particularly in biochemistry, molecular biology, and environmental science. This solution is meticulously prepared by dissolving ammonium phosphate monobasic (NH4H2PO4) in water to achieve the desired concentration for specific experimental purposes. In scientific research, ammonium phosphate monobasic solution plays several critical roles due to its unique properties and functionalities. One of its primary applications is as a buffering agent in biochemical and physiological assays. Ammonium phosphate monobasic acts as a weak acid, aiding in the maintenance of a stable pH environment within a defined range, typically around pH 4-7. This pH range is optimal for various enzymatic reactions, protein interactions, and cell culture experiments, making ammonium phosphate monobasic solution indispensable in laboratory protocols requiring precise pH control. Moreover, ammonium phosphate monobasic solution finds applications in molecular biology techniques, including nucleic acid isolation, purification, and electrophoresis. It is often utilized in conjunction with other buffering agents to prepare buffer systems for DNA and RNA extraction protocols. Additionally, ammonium phosphate monobasic solution serves as a component in agarose and polyacrylamide gel electrophoresis, providing ionic conductivity for the separation of nucleic acid fragments based on size. Furthermore, ammonium phosphate monobasic solution is employed in environmental research and water treatment processes. It is used as a nutrient source in microbiological culture media for the cultivation of microorganisms, particularly bacteria and fungi. Additionally, ammonium phosphate monobasic solution is utilized in wastewater treatment as a source of phosphorus, aiding in the removal of pollutants and the promotion of microbial activity for bioremediation purposes.