Molecular structure of Ammonium 7-Fluoro-2,1,3-benzoxadiazole-4-sulfonate, CAS Number: 84806-27-9
Ammonium 7-Fluoro-2,1,3-benzoxadiazole-4-sulfonate (CAS 84806-27-9)
Alternate Names:
7-Fluorobenzofurazan-4-sulfonic acid ammonium salt
Application:
Ammonium 7-Fluoro-2,1,3-benzoxadiazole-4-sulfonate is a bifunctional fluorogenic reagent
CAS Number:
84806-27-9
Molecular Weight:
235.20
Molecular Formula:
C6H6FN3O4S
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
Ammonium 7-Fluoro-2,1,3-benzoxadiazole-4-sulfonate is a bifunctional fluorogenic reagent used to detect thiols in biological specimens and proteins. Ammonium 7-Fluoro-2,1,3-benzoxadiazole-4-sulfonate does not contain any fluorescence but relies on its high reactivity to thiols to attain low detection limits. In addition, liquid chromatography is useful in analyzing low molecular weight thiols by pre-column derivatization with Ammonium 7-Fluoro-2,1,3-benzoxadiazole-4-sulfonate, which is followed by fluorometeric detection at 515nm. Furthermore, the addition of tri-n-butylphosphine (TBP) enables Ammonium 7-Fluoro-2,1,3-benzoxadiazole-4-sulfonate to react with thiols in soft metals.
Ammonium 7-Fluoro-2,1,3-benzoxadiazole-4-sulfonate (CAS 84806-27-9) References
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- Fluorogenic derivatization reagents suitable for isolation and identification of cysteine-containing proteins utilizing high-performance liquid chromatography-tandem mass spectrometry. | Masuda, M., et al. 2004. Anal Chem. 76: 728-35. PMID: 14750869
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- Determination of thiols and disulfides in normal rat tissues and hamster pancreas treated with N-nitrosobis(2-oxopropyl)amine using 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole and ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate. | Toyo'oka, T., et al. 1989. Biomed Chromatogr. 3: 166-72. PMID: 2590728
- Optimization of tris(2-carboxyethyl) phosphine reduction conditions for fast analysis of total biothiols in mouse serum samples. | Chang, C., et al. 2019. Heliyon. 5: e01598. PMID: 31193090
- Analysis of Total Thiols in the Urine of a Cystathionine β-Synthase-Deficient Mouse Model of Homocystinuria Using Hydrophilic Interaction Chromatography. | Chang, CF., et al. 2020. Molecules. 25: PMID: 32283791
- Quantification of Intracellular Thiols by HPLC-Fluorescence Detection. | Yamamoto, H., et al. 2021. Molecules. 26: PMID: 33921678
- High-performance liquid chromatography and fluorometric detection of biologically important thiols, derivatized with ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate (SBD-F). | Toyo'oka, T. and Imai, K. 1983. J Chromatogr. 282: 495-500. PMID: 6671013
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ammonium 7-Fluoro-2,1,3-benzoxadiazole-4-sulfonate, 25 mg | sc-210805 | 25 mg | $294.00 |