alpha 1A-AR Antibody (4D8) recommended for detection of α1A-AR of mouse, rat and human origin by WB, IP and ELISA

α1A-AR Antibody (4D8): sc-100291

α1A-AR Antibody (4D8) is rated 4.7 out of 5 by 3.
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Datasheets

    • Anti-α1A-AR Antibody (4D8) is a mouse monoclonal IgG1 (kappa light chain) α1A-AR antibody provided at 100 µg/ml
    • raised against an N-terminal region of α1A-AR of human origin
    • recommended for detection of α1A-AR of mouse, rat and human origin by WB, IP and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of α1A-AR (4D8): sc-100291.

How selective is this antibody for alpha-1A? We need to address the selectivity issue in light of Michel et al. (2009) "How reliable are G-protein-coupled receptor antibodies?" NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY 379: 386-388.

Asked by: cpoon
Thank you for your question. The manuscript you mention recommends validating by IHC, but I would like to aslso suggest that also using siRNAs and CRISPR followed by a WB would also provide validation results to determine the reliability of the antibody. You can find recommended gene silencing products on the antibody product block: https://www.scbt.com/scbt/product/alpha1a-ar-antibody-4d8?requestFrom=search If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-06-28

For Western Blot, is it recommended to use denatured or non-denatured conditions with α1A-AR (4D8): sc-100291 antibody?

Asked by: Germaine
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from Worked very well I used this antibody on mice tissues (liver, muscle, aorta) and it worked very well.
Date published: 2018-04-06
Rated 5 out of 5 by from positive results Product sc-100291 is used for kinase assay using human myeloid cells,positive results!
Date published: 2016-12-17
Rated 4 out of 5 by from Distinct band observed by WB using HL Distinct band observed by WB using HL-60 whole cell lysate. -SCBT QC
Date published: 2014-02-27
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