α-Syntrophin Antibody (D-7): sc-166634

α-Syntrophin Antibody (D-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects α-Syntrophin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). α-Syntrophin (D-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. α-Syntrophin plays a crucial role in organizing the dystrophin-associated protein complex, which is essential for maintaining muscle cell integrity. α-Syntrophin (D-7) monoclonal antibody recognizes proteins that interact with signaling molecules like nitric oxide synthase 1 (NOS1), facilitating their recruitment to the muscle sarcolemma and influencing synapse formation and neuromuscular junction stability. This interaction is vital for proper muscle function and signaling, as disruptions can lead to neuromuscular disorders. The syntrophin family, including α-Syntrophin, is characterized by PDZ domains, which are critical for protein-protein interactions, allowing them to serve as scaffolding proteins that organize various signaling pathways. Using anti-α-Syntrophin antibody (D-7) in research can provide insights into molecular mechanisms underlying muscle physiology and pathophysiology.

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α-Syntrophin Antibody (D-7) References:

1. Phosphorylation of dystrophin and alpha-syntrophin by Ca(2+)-calmodulin dependent protein kinase II.  |  Madhavan, R. and Jarrett, HW. 1999. Biochim Biophys Acta. 1434: 260-74. PMID: 10525145
2. Brazilian family with pure autosomal dominant spastic paraplegia maps to 8q: analysis of muscle beta 1 syntrophin.  |  Rocco, P., et al. 2000. Am J Med Genet. 92: 122-7. PMID: 10797436
3. Absence of alpha-syntrophin leads to structurally aberrant neuromuscular synapses deficient in utrophin.  |  Adams, ME., et al. 2000. J Cell Biol. 150: 1385-98. PMID: 10995443
4. Neuronal nitric oxide synthase localizes through multiple structural motifs to the sarcolemma in mouse myotubes.  |  Abdelmoity, A., et al. 2000. FEBS Lett. 482: 65-70. PMID: 11018524
5. The receptor tyrosine phosphatase-like protein ICA512 binds the PDZ domains of beta2-syntrophin and nNOS in pancreatic beta-cells.  |  Ort, T., et al. 2000. Eur J Cell Biol. 79: 621-30. PMID: 11043403
6. Alternative splicing of dystrobrevin regulates the stoichiometry of syntrophin binding to the dystrophin protein complex.  |  Newey, SE., et al. 2000. Curr Biol. 10: 1295-8. PMID: 11069112
7. Laminin-alpha1 globular domains 3 and 4 induce heterotrimeric G protein binding to alpha-syntrophin's PDZ domain and alter intracellular Ca2+ in muscle.  |  Zhou, YW., et al. 2005. Am J Physiol Cell Physiol. 288: C377-88. PMID: 15385269
8. {alpha}-Syntrophin regulates ARMS localization at the neuromuscular junction and enhances EphA4 signaling in an ARMS-dependent manner.  |  Luo, S., et al. 2005. J Cell Biol. 169: 813-24. PMID: 15939763
9. Evaluation of the specificity of four commercially available antibodies to alpha-syntrophin.  |  Eisinger, K., et al. 2015. Anal Biochem. 484: 99-101. PMID: 26079703
10. The adaptor protein alpha-syntrophin regulates adipocyte lipid droplet growth.  |  Eisinger, K., et al. 2016. Exp Cell Res. 345: 100-7. PMID: 27242274
11. Alpha-syntrophin dependent expression of tubulin alpha 8 protein in hepatocytes.  |  Rein-Fischboeck, L., et al. 2018. J Physiol Biochem. 74: 511-521. PMID: 30033487
12. Distinct roles of the dystrophin-glycoprotein complex: α-dystrobrevin and α-syntrophin in the maintenance of the postsynaptic apparatus of the neuromuscular synapse.  |  Valenzuela, IMY., et al. 2022. Hum Mol Genet. 31: 2370-2385. PMID: 35157076

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-α-Syntrophin Antibody (D-7) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-α-Syntrophin Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

α-Syntrophin Antibody (D-7) Specifications

Alternate Product Name: α-Syntrophin

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 427-461

Epitope Target: α-Syntrophin

Epitope Species: human

Product Reactivity/Detection: α-Syntrophin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for α-Syntrophin Antibody (D-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin