Date published: 2026-4-8

1-800-457-3801

SCBT Portrait Logo
Seach Input

α-Amanitin (CAS 23109-05-9)

4.5(2)
Write a reviewAsk a question

See product citations (26)

Alternate Names:
α-Amanitin also known as α-Amanitine; 9,18-(Iminoethaniminoethaniminoethaniminomethano)pyrrolo[1′,2′:8,9][1,5,8,11,14]thiatetraazacyclooctadecino[18,17-b]indole Cyclic Peptide Deriv.;Cyclo[L-α-aspartyl-(4R)-4-hydroxy-L-prolyl-(4R)-4,5-dihydroxy-L-isoleucyl-6-hydroxy-2-mercapto-L-tryptophylglycyl-L-isoleucylglycyl-L-cysteinyl] Cyclic (4→8)-Thioether (R)-S-Oxide
Application:
α-Amanitin is a specific and potent inhibitor of eukaryotic RNA pol II and a mild inhibitor of RNA pol III
CAS Number:
23109-05-9
Purity:
≥90%
Molecular Weight:
918.97
Molecular Formula:
C39H54N10O14S
Supplemental Information:
This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

QUICK LINKS

α-Amanitin is the major toxic bicyclic octapeptide of the Amanita phalloides mushroom that has been used in biochemical research for decades. It is synthesized as a proprotein, on ribosomes, 34 to 35 amino acids in length and then cleaved at specific proline residues by an enzyme belonging to the prolyl oligopeptidase (POP) subfamily. α-Amanitin shows remarkable binding affinity for eukaryotic RNA polymerase II, slightly binds to RNA polymerase III, and shows no activity on RNA polymerase I. It has been used to determine which types of RNA polymerase are present in a given sample. α-Amanitin works by binding to the bridging helix of RNA polymerase II inhibiting the translocation of RNA and DNA needed to empty the site for the next round of synthesis, thereby slowing the rate of transcription by over 1000 fold. alpha-Amanitin is an inhibitor of Pol III.


α-Amanitin (CAS 23109-05-9) References

  1. Alpha-amanitin blocks translocation by human RNA polymerase II.  |  Gong, XQ., et al. 2004. J Biol Chem. 279: 27422-7. PMID: 15096519
  2. Fifty years of amanitin.  |  Wieland, T. and Faulstich, H. 1991. Experientia. 47: 1186-93. PMID: 1765129
  3. Jamming the ratchet of transcription.  |  Svetlov, V. and Nudler, E. 2008. Nat Struct Mol Biol. 15: 777-9. PMID: 18679430
  4. Ribosomal biosynthesis of the cyclic peptide toxins of Amanita mushrooms.  |  Walton, JD., et al. 2010. Biopolymers. 94: 659-64. PMID: 20564017
  5. Distribution and mechanism of α-amanitin tolerance in mycophagous Drosophila (Diptera: Drosophilidae).  |  Stump, AD., et al. 2011. Environ Entomol. 40: 1604-12. PMID: 22217779
  6. Antiproliferative effect of pHLIP-amanitin.  |  Moshnikova, A., et al. 2013. Biochemistry. 52: 1171-8. PMID: 23360641
  7. Genome activation in bovine embryos: review of the literature and new insights from RNA sequencing experiments.  |  Graf, A., et al. 2014. Anim Reprod Sci. 149: 46-58. PMID: 24975847
  8. Transcriptional control of unequal cleavage in early Tubifex embryos.  |  Aoki, M. and Shimizu, T. 2017. Dev Genes Evol. 227: 279-287. PMID: 28624889
  9. Total Synthesis of α- and β-Amanitin.  |  Lutz, C., et al. 2020. Angew Chem Int Ed Engl. 59: 11390-11393. PMID: 32091645
  10. Targeted immunotherapy for HER2-low breast cancer with 17p loss.  |  Li, Y., et al. 2021. Sci Transl Med. 13: PMID: 33568521
  11. Transcriptional firing represses bactericidal activity in cystic fibrosis airway neutrophils.  |  Margaroli, C., et al. 2021. Cell Rep Med. 2: 100239. PMID: 33948572
  12. Medical error in treatment of Amanita phalloides poisoning in pre-hospital care.  |  Smędra, A., et al. 2022. Scand J Trauma Resusc Emerg Med. 30: 20. PMID: 35305697
  13. Specific inhibition of nuclear RNA polymerase II by alpha-amanitin.  |  Lindell, TJ., et al. 1970. Science. 170: 447-9. PMID: 4918258
  14. Effect of alpha-amanitin on ribonucleic acid synthesis and on ribonucleic acid polymerase in mouse liver.  |  Stirpe, F. and Fiume, L. 1967. Biochem J. 103: 67P-68P. PMID: 6049380
  15. The toxic peptides from Amanita mushrooms.  |  Wieland, T. 1983. Int J Pept Protein Res. 22: 257-76. PMID: 6354951

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

α-Amanitin, 1 mg

sc-202440
1 mg
$269.00

α-Amanitin, 5 mg

sc-202440A
5 mg
$1050.00

Is this product light sensitive?

Asked by: chemicalsmg
Thank you for your question. α-Amanitin: sc-202440 should be protected from light.
Answered by: Chemical Support 3
Date published: 2017-02-24

Does the vial for this product have a rubber stop or a screw cap?

Asked by: hawkeye11
Thank you for your question. α-Amanitin: sc-202440 has a screw top cap.
Answered by: Chemical Support 3
Date published: 2017-02-24

What is the expected appearance?

Asked by: chemicalsmg
Thank you for your question. α-Amanitin: sc-202440 is expected to be a white to light yellow powder.
Answered by: Chemical Support 3
Date published: 2017-02-24
  • y_2026, m_3, d_26, h_7CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.43
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_en_US, sid_202440, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 98ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from This product is pretty effectiveThis product is pretty effective. I used it to inhibit RNA Pol II in MCF10A cells. The concentration I used was 50 ug/ml and treated the cells for 4 hrs. The 1 point I took off is because for this product (highly toxic), I would expect it comes with a rubber cap container so that I can inject certain amount of solvent without opening the cap. However, it came with a plastic cap. You can still inject your solvent to dissolve the powder, but with more carefulness.
Date published: 2015-09-23
Rated 5 out of 5 by from Nguyen et alNguyen et al. (PubMed ID 8760875) found alpha-amanitin irreversibly inhibited RNA polymerase II (RNAPII) and triggered degradation of the RPBI subunit of RNAPII. -SCBT Publication Review
Date published: 2015-02-07
  • y_2026, m_3, d_26, h_7
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.43
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_202440, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 11ms
  • REVIEWS, PRODUCT
α-Amanitin is rated 4.5 out of 5 by 2.
  • y_2026, m_3, d_26, h_7
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.43
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_202440, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 72ms
  • REVIEWS, PRODUCT