Date published: 2025-9-5

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α-Actin Antibody (Alpha Sr-1): sc-58671

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Datasheets
  • α-Actin Antibody (Alpha Sr-1) is a mouse monoclonal IgM κ, cited in 11 publications, provided at 200 µg/ml
  • recommended for detection of α-skeletal and α-cardiac Actin of mouse, rat, human and Xenopus origin by WB, IP, IF and IHC(P); also reactive with additional species, including and bovine and rabbit; not recommended for detection of α-Actin from smooth muscle tissue
  • See α-Actin (1A4): sc-32251 for α-Actin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of α-Actin (Alpha Sr-1): sc-58671.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for α-Actin Antibody (Alpha Sr-1). This work is in progress.

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α-Actin Antibody (Alpha Sr-1) is a mouse monoclonal IgM antibody that detects α-Actin from mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-α-Actin antibody (Alpha Sr-1) is available in non-conjugated form. Actin plays a vital role in various cellular functions, including maintaining cell shape, enabling motility, and facilitating muscle contraction, making α-Actin essential for both structural integrity and dynamic cellular processes. α-Actin (Alpha Sr-1) antibody targets proteins predominantly located in muscle cells, where α-Actin forms the contractile apparatus, while also being present in other cell types as part of the cytoskeleton′s architecture. In mammals, α-Actin is specifically expressed in skeletal and cardiac muscle, distinguishing α-Actin from other isoforms such as β-Actin and γ-Actin found in non-muscle tissues. Multiple actin isoforms allow for specialized functions in different cell types, highlighting α-Actin′s importance in muscle physiology and regulation by small GTPases, which orchestrate the assembly and organization of the actin cytoskeleton. Anti-α-Actin antibody (Alpha Sr-1) serves as a valuable tool for researchers studying muscle development, function, and related pathologies, providing insights into actin′s intricate roles in health and disease.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

α-Actin Antibody (Alpha Sr-1)

sc-58671
200 µg/ml
$316.00

(Alpha Sr-1): sc-58671 , can i know the specific epitope of this antibody?

Asked by: oneone
Thank you for your question. a-Actin (Alpha Sr-1) is a mouse monoclonal antibody raised against Actin of rabbit origin. The exact epitope of this antibody wasn't characterized.
Answered by: Technical Service SH
Date published: 2023-07-24

I am using α-Actin (Alpha Sr-1): sc-58671 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Worked well for IF staining of PFA fixed rat heartWorked well for IF staining of PFA fixed rat heart. -SCBT Publication Review
Date published: 2011-10-28
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α-Actin Antibody (Alpha Sr-1) is rated 4.0 out of 5 by 1.
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