alpha-Actin Antibody (alpha-SM1): sc-130617

α-Actin Antibody (alpha-SM1) is a mouse monoclonal IgG2a antibody that detects α-Actin in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). α-Actin is a vital component of the cytoskeleton, playing a crucial role in maintaining cell shape, enabling cellular movement, and facilitating muscle contraction. α-Actin is predominantly found in muscle tissues, where α-Actin forms part of the contractile apparatus, but α-Actin is also present in non-muscle cells, contributing to the structural integrity of the cell. The presence of multiple isoforms of Actin, such as α-, β-, and γ-Actin, allows for specialized functions in different cell types, with α-Actin being essential for muscle function. The regulation of α-Actin is influenced by various signaling pathways, including those involving small GTPases, which orchestrate the dynamic organization of the Actin cytoskeleton. Understanding the function and regulation of α-Actin is critical, as alterations in α-Actin expression or organization can lead to various diseases, including cardiomyopathies and other muscle-related disorders. With broad reactivity and versatility in applications, anti-α-Actin antibody (alpha-SM1) is an invaluable tool for researchers studying the intricate roles of Actin in cellular processes.

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alpha-Actin Antibody (alpha-SM1) References:

1. Alpha-actin isoform distribution in normal and failing human heart: a morphological, morphometric, and biochemical study.  |  Suurmeijer, AJ., et al. 2003. J Pathol. 199: 387-97. PMID: 12579541
2. Smooth Muscle alpha-actin is a direct target of Notch/CSL.  |  Noseda, M., et al. 2006. Circ Res. 98: 1468-70. PMID: 16741155
3. Mutations in smooth muscle alpha-actin (ACTA2) lead to thoracic aortic aneurysms and dissections.  |  Guo, DC., et al. 2007. Nat Genet. 39: 1488-93. PMID: 17994018
4. The vascular smooth muscle alpha-actin gene is reactivated during cardiac hypertrophy provoked by load.  |  Black, FM., et al. 1991. J Clin Invest. 88: 1581-8. PMID: 1834699
5. alpha-Actin proteins and gene transcripts are colocalized in embryonic mouse muscle.  |  Lyons, GE., et al. 1991. Development. 111: 451-4. PMID: 1893870
6. Mutations and polymorphisms of the skeletal muscle alpha-actin gene (ACTA1).  |  Laing, NG., et al. 2009. Hum Mutat. 30: 1267-77. PMID: 19562689
7. Nemaline myopathy-related skeletal muscle α-actin (ACTA1) mutation, Asp286Gly, prevents proper strong myosin binding and triggers muscle weakness.  |  Ochala, J., et al. 2012. PLoS One. 7: e45923. PMID: 23029319
8. alpha-actin down regulation and perforin loss in uterine natural killer cells from LPS-treated pregnant mice.  |  Zavan, B., et al. 2015. Physiol Res. 64: 427-32. PMID: 26066976
9. Cooperative signaling by TGF-β1 and WNT-11 drives sm-α-actin expression in smooth muscle via Rho kinase-actin-MRTF-A signaling.  |  Kumawat, K., et al. 2016. Am J Physiol Lung Cell Mol Physiol. 311: L529-37. PMID: 27422998
10. A common factor regulates skeletal and cardiac alpha-actin gene transcription in muscle.  |  Muscat, GE., et al. 1988. Mol Cell Biol. 8: 4120-33. PMID: 3185543

The preferred α-Actin antibody is α-Actin Antibody (1A4): sc-32251.