alpha-Actin Antibody (a-SM1) recommended for detection of smooth muscle α-Actin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

α-Actin Antibody (a-SM1): sc-130616

α-Actin Antibody (a-SM1) is rated 4.0 out of 5 by 2.
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Datasheets

    • α-Actin Antibody (a-SM1) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against an N-terminal decapeptide of smooth muscle α-Actin of human origin
    • recommended for detection of smooth muscle α-Actin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; non cross-reactive with actin from fibroblasts (β-and γ-cytoplasmic), myocardium (α-myocardial), and striated muscle (α-sarcomeric)
    • available conjugated to agarose for IP
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See α-Actin (1A4): sc-32251 for α-Actin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

What is the recommended secondary for this mouse monoclonal primary?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-02-03
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Rated 4 out of 5 by from Good results in IF with induced pluripotent Good results in IF with induced pluripotent stem cells (m-iPSC) -SCBT Publication Review
Date published: 2012-03-19
Rated 4 out of 5 by from Works well in pig heart Works well in pig heart. -SCBT Publication Review
Date published: 2009-06-18
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