ALDH1L2 CRISPR/Cas9 KO Plasmid (h): sc-406648
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ALDH1L2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ALDH1L2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ALDH1L2 CRISPR/Cas9 KO Plasmid (h) | sc-406648 | 20 µg | $397.00 |
Overview
ALDH1L2 encodes a mitochondrial aldehyde dehydrogenase that functions as a 10-formyltetrahydrofolate dehydrogenase, supporting one-carbon folate metabolism and redox homeostasis within mitochondria. By regulating formyl-THF turnover and NADP(H)-linked reactions, ALDH1L2 influences nucleotide biosynthesis capacity, mitochondrial oxidative balance, and cellular responses to metabolic stress. Perturbation of mitochondrial one-carbon flux can reshape anabolic demands and reactive oxygen species handling, processes frequently altered in proliferative and stress-adapted cell states. Accordingly, ALDH1L2 is studied in the context of metabolic rewiring, mitochondrial function, and disease-associated changes in folate-dependent pathways.
ALDH1L2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ALDH1L2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ALDH1L2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ALDH1L2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ALDH1L2 protein expression.
This CRISPR knockout system enables efficient generation of ALDH1L2-deficient cell models for investigation of ALDH1L2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ALDH1L2 exon(s) critical for ALDH1L2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ALDH1L2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ALDH1L2 CRISPR/Cas9 KO Plasmid (h) and ALDH1L2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ALDH1L2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ALDH1L2 HDR Plasmid (h) and ALDH1L2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ALDH1L2 homology arms to support homology-directed repair at defined ALDH1L2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.