Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Aldehyde dehydrogenase 3-A1/ALDH3A1 Double Nickase Plasmid (h): sc-402168-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Aldehyde dehydrogenase 3-A1/ALDH3A1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Aldehyde dehydrogenase 3-A1/ALDH3A1 Double Nickase Plasmid (h) and Aldehyde dehydrogenase 3-A1/ALDH3A1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ALDH3A1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Aldehyde dehydrogenase 3-A1/ALDH3A1 Antibody (G-2): sc-376089
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aldehyde dehydrogenase 3-A1/ALDH3A1 Double Nickase Plasmid (h) | sc-402168-NIC | 20 µg | $410.00 | |||
Aldehyde dehydrogenase 3-A1/ALDH3A1 Double Nickase Plasmid (h2) | sc-402168-NIC-2 | 20 µg | $410.00 |
ALDH3A1 encodes aldehyde dehydrogenase 3A1, a cytosolic NAD(P)+-dependent enzyme that oxidizes reactive aliphatic and aromatic aldehydes to their corresponding carboxylic acids. By detoxifying lipid peroxidation byproducts and exogenous aldehydes, ALDH3A1 supports redox homeostasis, limits aldehyde-protein adduct formation, and contributes to cellular defense against oxidative and electrophilic stress. Its activity intersects with broader aldehyde metabolism, glutathione-dependent antioxidant networks, and stress-response transcriptional programs. Altered ALDH3A1 expression or function has been investigated in contexts involving epithelial stress adaptation, inflammation-associated damage, and carcinogenesis-related metabolic remodeling.
Aldehyde dehydrogenase 3-A1/ALDH3A1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ALDH3A1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ALDH3A1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ALDH3A1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ALDH3A1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.